A Human Cellular Model for Colorectal Anastomotic Repair: The Effect of Localization and Transforming Growth Factor-β1 Treatment on Collagen Deposition and Biomarkers.
Anastomosis, Surgical
/ adverse effects
Anastomotic Leak
/ surgery
Biomarkers
/ metabolism
Cells, Cultured
Collagen
/ genetics
Colon
/ cytology
Colorectal Surgery
/ adverse effects
Culture Media, Conditioned
/ chemistry
Female
Fibroblasts
/ cytology
Flow Cytometry
Humans
Male
Models, Biological
Primary Cell Culture
Rectum
/ cytology
Transforming Growth Factor beta1
/ pharmacology
anastomotic leakage
collagen
colon
extracellular matrix
growth factors
rectum
wound healing
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
05 Feb 2021
05 Feb 2021
Historique:
received:
18
01
2021
revised:
02
02
2021
accepted:
03
02
2021
entrez:
10
2
2021
pubmed:
11
2
2021
medline:
3
9
2021
Statut:
epublish
Résumé
Anastomotic leakage (AL) is a devastating complication after colorectal surgery, possibly due to the loss of stabilizing collagen fibers in the submucosa. Our aim was to assess the formation of collagen in the colon versus the rectum with or without transforming growth factor (TGF)-β1 exposure in a human cellular model of colorectal repair. Primary fibroblasts were isolated by an explant procedure from clinically resected tissue rings during anastomosis construction in 19 consecutive colorectal patients who underwent laparoscopy. The cells, identified as fibroblasts by morphologic characteristics and flow cytometry analysis (CD90
Identifiants
pubmed: 33562728
pii: ijms22041616
doi: 10.3390/ijms22041616
pmc: PMC7914853
pii:
doi:
Substances chimiques
Biomarkers
0
Culture Media, Conditioned
0
Transforming Growth Factor beta1
0
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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