NMR structure and dynamics of Q4DY78, a conserved kinetoplasid-specific protein from Trypanosoma cruzi.
Amino Acid Sequence
Binding Sites
Calcium
/ metabolism
Cell Adhesion Molecules
/ chemistry
Circular Dichroism
Conserved Sequence
EF Hand Motifs
Microfilament Proteins
/ chemistry
Models, Molecular
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular
Phosphoproteins
/ chemistry
Protein Conformation, alpha-Helical
Protein Structure, Secondary
Protozoan Proteins
/ chemistry
Trypanosoma cruzi
/ chemistry
Drug target
Kinetoplastids
NMR
Neglected diseases
Protein structure
Trypanosoma cruzi
Journal
Journal of structural biology
ISSN: 1095-8657
Titre abrégé: J Struct Biol
Pays: United States
ID NLM: 9011206
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
06
10
2020
revised:
01
03
2021
accepted:
03
03
2021
pubmed:
12
3
2021
medline:
27
1
2022
entrez:
11
3
2021
Statut:
ppublish
Résumé
The 106-residue protein Q4DY78 (UniProt accession number) from Trypanosoma cruzi is highly conserved in the related kinetoplastid pathogens Trypanosoma brucei and Leishmania major. Given the essentiality of its orthologue in T. brucei, the high sequence conservation with other trypanosomatid proteins, and the low sequence similarity with mammalian proteins, Q4DY78 is an attractive protein for structural characterization. Here, we solved the structure of Q4DY78 by solution NMR and evaluated its backbone dynamics. Q4DY78 is composed of five α -helices and a small, two-stranded antiparallel β-sheet. The backbone RMSD is 0.22 ± 0.05 Å for the representative ensemble of the 20 lowest-energy structures. Q4DY78 is overall rigid, except for N-terminal residues (V
Identifiants
pubmed: 33705979
pii: S1047-8477(21)00020-4
doi: 10.1016/j.jsb.2021.107715
pii:
doi:
Substances chimiques
Cell Adhesion Molecules
0
Microfilament Proteins
0
Nitrogen Isotopes
0
Nitrogen-15
0
Phosphoproteins
0
Protozoan Proteins
0
vasodilator-stimulated phosphoprotein
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107715Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.