Seedling developmental defects upon blocking CINNAMATE-4-HYDROXYLASE are caused by perturbations in auxin transport.
Arabidopsis
auxin
cis-cinnamic acid
lignin
metabolomics
phenylpropanoids
piperonylic acid
roots
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
15
01
2021
accepted:
06
03
2021
pubmed:
18
3
2021
medline:
5
6
2021
entrez:
17
3
2021
Statut:
ppublish
Résumé
The phenylpropanoid pathway serves a central role in plant metabolism, providing numerous compounds involved in diverse physiological processes. Most carbon entering the pathway is incorporated into lignin. Although several phenylpropanoid pathway mutants show seedling growth arrest, the role for lignin in seedling growth and development is unexplored. We use complementary pharmacological and genetic approaches to block CINNAMATE-4-HYDROXYLASE (C4H) functionality in Arabidopsis seedlings and a set of molecular and biochemical techniques to investigate the underlying phenotypes. Blocking C4H resulted in reduced lateral rooting and increased adventitious rooting apically in the hypocotyl. These phenotypes coincided with an inhibition in AUX transport. The upstream accumulation in cis-cinnamic acid was found to be likely to cause polar AUX transport inhibition. Conversely, a downstream depletion in lignin perturbed phloem-mediated AUX transport. Restoring lignin deposition effectively reestablished phloem transport and, accordingly, AUX homeostasis. Our results show that the accumulation of bioactive intermediates and depletion in lignin jointly cause the aberrant phenotypes upon blocking C4H, and demonstrate that proper deposition of lignin is essential for the establishment of AUX distribution in seedlings. Our data position the phenylpropanoid pathway and lignin in a new physiological framework, consolidating their importance in plant growth and development.
Substances chimiques
Cinnamates
0
Indoleacetic Acids
0
Trans-Cinnamate 4-Monooxygenase
EC 1.14.14.91
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2275-2291Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L009366/1
Pays : United Kingdom
Informations de copyright
© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
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