An RNA-binding protein complex regulates the purine-dependent expression of a nucleobase transporter in trypanosomes.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
19 04 2021
19 04 2021
Historique:
accepted:
04
03
2021
revised:
02
03
2021
received:
23
05
2020
pubmed:
22
3
2021
medline:
13
5
2021
entrez:
21
3
2021
Statut:
ppublish
Résumé
Post-transcriptional regulation of gene expression is particularly important in trypanosomatid protozoa. RNA-binding proteins (RBPs) regulate mRNA stability and translation, yet information about how RBPs are able to link environmental cues to post-transcriptional control is scarce. In Trypanosoma brucei, we have previously characterized a short RNA stem-loop cis-element (PuRE, Purine Responsive Element) within the 3'-UTR of the NT8 nucleobase transporter mRNA that is necessary and sufficient to confer a strong repression of gene expression in response to purines. In this study, we have identified a protein complex composed of two RNA-binding proteins (PuREBP1 and PuREBP2) that binds to the PuRE in vitro and to NT8 mRNA in vivo. Depletion of PuREBP1 by RNA interference results in the upregulation of just NT8 and the mRNAs encoding the amino acid transporter AATP6 paralogues. Moreover, we found that the PuREBP1/2 complex is associated with only a handful of mRNAs, and that it is responsible for the observed purine-dependent regulation of NT8 expression.
Identifiants
pubmed: 33744953
pii: 6179354
doi: 10.1093/nar/gkab181
pmc: PMC8053114
doi:
Substances chimiques
3' Untranslated Regions
0
Protozoan Proteins
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3814-3825Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Mol Microbiol. 2016 May;100(3):457-71
pubmed: 26784394
Methods. 2001 Jul;24(3):218-29
pubmed: 11403571
Wiley Interdiscip Rev RNA. 2018 May 31;:e1485
pubmed: 29851258
Nucleic Acids Res. 2013 May 1;41(10):e108
pubmed: 23558742
BMC Genomics. 2010 May 05;11:283
pubmed: 20444260
Mol Biochem Parasitol. 1996 May;77(2):235-9
pubmed: 8813669
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
PLoS Pathog. 2014 Feb 27;10(2):e1003938
pubmed: 24586154
Mol Biochem Parasitol. 2007 Jul;154(1):103-9
pubmed: 17512617
Mol Biochem Parasitol. 1995 Dec;75(1):1-14
pubmed: 8720170
Mol Cell. 2018 Jun 7;70(5):854-867.e9
pubmed: 29883606
Mol Biochem Parasitol. 2006 Sep;149(1):1-9
pubmed: 16682088
Bioinformatics. 2014 Apr 1;30(7):923-30
pubmed: 24227677
J Biol Chem. 2020 Jun 19;295(25):8449-8459
pubmed: 32354744
J Clin Invest. 2008 Apr;118(4):1301-10
pubmed: 18382742
PLoS Pathog. 2014 Jun 12;10(6):e1004178
pubmed: 24945722
PLoS One. 2012;7(11):e48870
pubmed: 23145003
Mol Biochem Parasitol. 2000 Feb 25;106(1):93-107
pubmed: 10743614
Eukaryot Cell. 2005 Dec;4(12):2106-14
pubmed: 16339728
Trends Parasitol. 2011 Jan;27(1):23-30
pubmed: 20609625
Curr Opin Microbiol. 2016 Aug;32:46-51
pubmed: 27177350
PLoS Pathog. 2009 Aug;5(8):e1000565
pubmed: 19714224
Nat Biotechnol. 2019 Feb;37(2):169-178
pubmed: 30607034
FEMS Microbiol Rev. 2005 Nov;29(5):987-1020
pubmed: 16040150
Nucleic Acids Res. 2008 Aug;36(14):4573-86
pubmed: 18611951
PLoS One. 2012;7(5):e36619
pubmed: 22574199
Mol Biochem Parasitol. 2003 Aug 31;130(2):101-10
pubmed: 12946846
Open Biol. 2019 Jun 28;9(6):190072
pubmed: 31164043
Nature. 2014 Jan 30;505(7485):681-685
pubmed: 24336212
Eukaryot Cell. 2004 Apr;3(2):245-54
pubmed: 15075255
Future Microbiol. 2011 Apr;6(4):459-74
pubmed: 21526946
Genome Res. 2020 Jul;30(7):962-973
pubmed: 32703884
PLoS One. 2018 Dec 31;13(12):e0206332
pubmed: 30596656
PLoS Pathog. 2017 Mar 3;13(3):e1006245
pubmed: 28257521
Methods Mol Biol. 2005;309:39-60
pubmed: 15990397
EMBO J. 2001 Jul 16;20(14):3831-9
pubmed: 11447124
Wiley Interdiscip Rev RNA. 2020 May;11(3):e1582
pubmed: 31883228
Nature. 2013 Jul 11;499(7457):172-7
pubmed: 23846655
Mol Biochem Parasitol. 1997 Mar;85(1):99-112
pubmed: 9108552
RNA. 2012 Nov;18(11):1968-83
pubmed: 22966087
Nucleic Acids Res. 2014 Jun;42(11):7201-9
pubmed: 24813448
Mol Microbiol. 2016 Jul;101(2):299-313
pubmed: 27062185
RNA. 2010 Aug;16(8):1673-8
pubmed: 20571124
Nucleic Acids Res. 2019 Jul 26;47(13):7063-7077
pubmed: 31127277
Curr Opin Genet Dev. 2018 Feb;48:97-103
pubmed: 29175729
Elife. 2014 Jun 16;3:
pubmed: 24935936
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Mol Cell. 2003 May;11(5):1291-9
pubmed: 12769852
Science. 2012 Dec 7;338(6112):1352-3
pubmed: 23224556
Trends Parasitol. 2017 Feb;33(2):80-82
pubmed: 27863903
Wiley Interdiscip Rev RNA. 2010 Jul-Aug;1(1):34-46
pubmed: 21956905
Acta Trop. 1979 Sep;36(3):289-92
pubmed: 43092
PLoS Pathog. 2017 Aug 11;13(8):e1006560
pubmed: 28800584
PLoS Negl Trop Dis. 2012;6(9):e1833
pubmed: 23050028