Identification of the PP2C gene family in paper mulberry (Broussonetia papyrifera) and its roles in the regulation mechanism of the response to cold stress.
Broussonetia
/ classification
Chromosome Mapping
Cold-Shock Response
/ genetics
Gene Duplication
Gene Expression
Gene Expression Regulation, Plant
Genes, Plant
Genome, Plant
/ genetics
Multigene Family
Phosphoprotein Phosphatases
/ genetics
Phosphorylation
Phylogeny
Plant Proteins
/ genetics
Promoter Regions, Genetic
Protein Domains
Protein Interaction Maps
Signal Transduction
Synteny
Cold stress
Paper mulberry
Phylogenetic analysis
Protein-protein network
Type-2C protein phosphatase (PP2C)
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
27
07
2020
accepted:
27
02
2021
pubmed:
23
3
2021
medline:
6
11
2021
entrez:
22
3
2021
Statut:
ppublish
Résumé
To study the possible roles of type-2C protein phosphatases (PP2Cs) which have been confirmed to play roles in the response to diverse abiotic stresses in paper mulberry, we launched a series of genomic and functional studies of BpPP2Cs. Sixty-three PP2C proteins in paper mulberry (Broussonetia papyrifera) were classified into 13 clades. Four BpPP2Cs with kinase domains were verified to be highly conserved in organisms ranging from algae to dicots. Seven pairs of BpPP2C genes were found to be expanding, and 18 BpPP2C genes had orthologues in Arabidopsis. BpPP2Cs showed broad expression in different tissues; the expression levels of 18 BpPP2Cs were changed and the phosphorylation levels of seven BpPP2C proteins increased at low temperature. Cold-response elements were found in the promoter region of 31 BpPP2Cs. Finally, Bp01g0320 was found to act as a hub protein and Bp01g0512 and Bp09g1278 played key roles in the ABA-signaling pathway and MAPK cascades, respectively. These results suggest that the PP2C gene family of paper mulberry is evolutionarily conserved and participates the regulation of the response to cold stress, which will play a vital role in further research on phosphatases in paper mulberry.
Identifiants
pubmed: 33751277
doi: 10.1007/s10529-021-03110-4
pii: 10.1007/s10529-021-03110-4
doi:
Substances chimiques
Plant Proteins
0
Phosphoprotein Phosphatases
EC 3.1.3.16
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1089-1102Subventions
Organisme : National Natural Science Foundation of China
ID : 31870247
Organisme : Beijing Natural Science Foundation
ID : 6202026
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