Mechanistic Insights into Substrate Recognition and Catalysis of a New Ulvan Lyase of Polysaccharide Lyase Family 24.

Alteromonadaceae / enzymology Amino Acid Sequence Catalysis Phylogeny Polysaccharide-Lyases / chemistry Polysaccharides / chemistry Substrate Specificity
catalytic mechanism marine bacterium polysaccharide lyase family 24 substrate recognition ulvan ulvan lyase

Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
26 05 2021
Historique:
pubmed: 28 3 2021
medline: 4 9 2021
entrez: 27 3 2021
Statut: ppublish

Résumé

Ulvan is an important marine polysaccharide. Bacterial ulvan lyases play important roles in ulvan degradation and marine carbon cycling. Until now, only a small number of ulvan lyases have been characterized. Here, a new ulvan lyase, Uly1, belonging to polysaccharide lyase family 24 (PL24) from the marine bacterium Catenovulum maritimum, is characterized. The optimal temperature and pH for Uly1 to degrade ulvan are 40°C and pH 9.0, respectively. Uly1 degrades ulvan polysaccharides in the endolytic manner, mainly producing ΔRha3S, consisting of an unsaturated 4-deoxy-l-

Identifiants

DOI: 10.1128/AEM.00412-21 PMID: 33771786 PMC: PMC8174760
pubmed: 33771786
pii: AEM.00412-21
doi: 10.1128/AEM.00412-21
pmc: PMC8174760
doi:

Substances chimiques

Polysaccharides 0
ulvan 0
Polysaccharide-Lyases EC 4.2.2.-
ulvan-lyase EC 4.2.2.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0041221

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Auteurs

Fei Xu (F)

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Fang Dong (F)

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Xiao-Hui Sun (XH)

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Hai-Yan Cao (HY)

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Hui-Hui Fu (HH)

College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China.

Chun-Yang Li (CY)

College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China.

Xi-Ying Zhang (XY)

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Andrew McMinn (A)

College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China.
Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, Tasmania, Australia.

Yu-Zhong Zhang (YZ)

College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China.
Marine Biotechnology Research Center, Shandong University, Qingdao, China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Peng Wang (P)

College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China.

Xiu-Lan Chen (XL)

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, China.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Alteromonadaceae › Mechanistic Insights into Substrate...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Mechanistic Insights into Substrate...
questionsmedicales.fr › Phénomènes chimiques › Catalyse › Mechanistic Insights into Substrate...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Mechanistic Insights into Substrate...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-oxygen lyases › Polysaccharide-lyases › Mechanistic Insights into Substrate...
questionsmedicales.fr › Glucides › Polyosides › Mechanistic Insights into Substrate...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Mechanistic Insights into Substrate...