Transport of UDP-rhamnose by URGT2, URGT4, and URGT6 modulates rhamnogalacturonan-I length.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
02 04 2021
02 04 2021
Historique:
received:
03
08
2020
accepted:
19
11
2020
pubmed:
2
4
2021
medline:
6
8
2021
entrez:
1
4
2021
Statut:
ppublish
Résumé
Rhamnogalacturonan-I biosynthesis occurs in the lumen of the Golgi apparatus, a compartment where UDP-Rhamnose and UDP-Galacturonic Acid are the main substrates for synthesis of the backbone polymer of pectin. Recent studies showed that UDP-Rha is transported from the cytosol into the Golgi apparatus by a family of six UDP-rhamnose/UDP-galactose transporters (URGT1-6). In this study, analysis of adherent and soluble mucilage (SM) of Arabidopsis thaliana seeds revealed distinct roles of URGT2, URGT4, and URGT6 in mucilage biosynthesis. Characterization of SM polymer size showed shorter chains in the urgt2 urgt4 and urgt2 urgt4 urgt6 mutants, suggesting that URGT2 and URGT4 are mainly involved in Rhamnogalacturonan-I (RG-I) elongation. Meanwhile, mutants in urgt6 exhibited changes only in adherent mucilage (AM). Surprisingly, the estimated number of RG-I polymer chains present in urgt2 urgt4 and urgt2 urgt4 urgt6 mutants was higher than in wild-type. Interestingly, the increased number of shorter RG-I chains was accompanied by an increased amount of xylan. In the urgt mutants, expression analysis of other genes involved in mucilage biosynthesis showed some compensation. Studies of mutants of transcription factors regulating mucilage formation indicated that URGT2, URGT4, and URGT6 are likely part of a gene network controlled by these regulators and involved in RG-I synthesis. These results suggest that URGT2, URGT4, and URGT6 play different roles in the biosynthesis of mucilage, and the lack of all three affects the production of shorter RG-I polymers and longer xylan domains.
Identifiants
pubmed: 33793913
pii: 6054815
doi: 10.1093/plphys/kiaa070
pmc: PMC8133686
doi:
Substances chimiques
AT1G21070 protein, Arabidopsis
0
Arabidopsis Proteins
0
Monosaccharide Transport Proteins
0
Rhamnogalacturonans
0
rhamnogalacturonan I
0
Pectins
89NA02M4RX
N-Glycosyl Hydrolases
EC 3.2.2.-
URH1 protein, Arabidopsis
EC 3.2.2.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
914-933Commentaires et corrections
Type : CommentIn
Informations de copyright
© American Society of Plant Biologists 2020. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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