Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo.

Animals Blastocyst / cytology Cell Differentiation Cell Lineage Cells, Cultured Chimerism Embryo, Mammalian / cytology Female Humans Macaca fascicularis Pluripotent Stem Cells / cytology RNA-Seq Single-Cell Analysis Transcriptome
ex-vivo-cultured mokey embryos human extended pluripotent stem cells human-monkey chimeric embryo interspecies chimera pluripotent stem cell

Journal

Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066

Informations de publication

Date de publication:
15 04 2021
Historique:
received: 29 09 2020
revised: 25 01 2021
accepted: 09 03 2021
entrez: 16 4 2021
pubmed: 17 4 2021
medline: 21 10 2021
Statut: ppublish

Résumé

Interspecies chimera formation with human pluripotent stem cells (hPSCs) represents a necessary alternative to evaluate hPSC pluripotency in vivo and might constitute a promising strategy for various regenerative medicine applications, including the generation of organs and tissues for transplantation. Studies using mouse and pig embryos suggest that hPSCs do not robustly contribute to chimera formation in species evolutionarily distant to humans. We studied the chimeric competency of human extended pluripotent stem cells (hEPSCs) in cynomolgus monkey (Macaca fascicularis) embryos cultured ex vivo. We demonstrate that hEPSCs survived, proliferated, and generated several peri- and early post-implantation cell lineages inside monkey embryos. We also uncovered signaling events underlying interspecific crosstalk that may help shape the unique developmental trajectories of human and monkey cells within chimeric embryos. These results may help to better understand early human development and primate evolution and develop strategies to improve human chimerism in evolutionarily distant species.

Identifiants

DOI: 10.1016/j.cell.2021.03.020 PMID: 33861963
pubmed: 33861963
pii: S0092-8674(21)00305-6
doi: 10.1016/j.cell.2021.03.020
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2020-2032.e14

Commentaires et corrections

Type : CommentIn
Type : CommentIn
Type : ErratumIn

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Tao Tan (T)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: tant@lpbr.cn.

Jun Wu (J)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address: jun2.wu@utsouthwestern.edu.

Chenyang Si (C)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Shaoxing Dai (S)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Youyue Zhang (Y)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Nianqin Sun (N)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

E Zhang (E)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Honglian Shao (H)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Wei Si (W)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Pengpeng Yang (P)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Hong Wang (H)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Zhenzhen Chen (Z)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Ran Zhu (R)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Yu Kang (Y)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Reyna Hernandez-Benitez (R)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Llanos Martinez Martinez (L)

Universidad Católica San Antonio de Murcia (UCAM), Campus de los Jerónimos, No 135 12, Guadalupe 30107, Spain.

Estrella Nuñez Delicado (E)

Universidad Católica San Antonio de Murcia (UCAM), Campus de los Jerónimos, No 135 12, Guadalupe 30107, Spain.

W Travis Berggren (WT)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

May Schwarz (M)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Zongyong Ai (Z)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Tianqing Li (T)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

Concepcion Rodriguez Esteban (C)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Weizhi Ji (W)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: wji@lpbr.cn.

Yuyu Niu (Y)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: niuyy@lpbr.cn.

Juan Carlos Izpisua Belmonte (JC)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA. Electronic address: belmonte@salk.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chimeric contribution of human extended...
questionsmedicales.fr › Structures de l'embryon › Embryon de mammifère › Blastocyste › Chimeric contribution of human extended...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Chimeric contribution of human extended...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Développement embryonnaire et foetal › Développement embryonnaire › Lignage cellulaire › Chimeric contribution of human extended...
questionsmedicales.fr › Cellules › Cellules cultivées › Chimeric contribution of human extended...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Aberrations des chromosomes › Chimérisme › Chimeric contribution of human extended...
questionsmedicales.fr › Structures de l'embryon › Embryon de mammifère › Chimeric contribution of human extended...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Chimeric contribution of human extended...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca › Macaca fascicularis › Chimeric contribution of human extended...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Chimeric contribution of human extended...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ARN › RNA-Seq › Chimeric contribution of human extended...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Chimeric contribution of human extended...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Transcriptome › Chimeric contribution of human extended...