Expression of peat moss VASCULAR RELATED NAC-DOMAIN homologs in Nicotiana benthamiana leaf cells induces ectopic secondary wall formation.
Cell Wall
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Plant
/ genetics
High-Throughput Nucleotide Sequencing
Plant Leaves
/ metabolism
Plant Proteins
/ genetics
Plants, Genetically Modified
/ genetics
Protein Domains
Sphagnopsida
/ genetics
Nicotiana
/ metabolism
Transcription Factors
/ genetics
Xylem
/ metabolism
Hyaline cell
Peat moss
Sphagnum palustre
VND
VNS
Water storage
Water-conducting
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
02
08
2020
accepted:
29
03
2021
pubmed:
22
4
2021
medline:
22
5
2021
entrez:
21
4
2021
Statut:
ppublish
Résumé
The homologs of VASCULAR RELATED NAC-DOMAIN in the peat moss Sphagnum palustre were identified and these transcriptional activity as the VNS family was conserved. In angiosperms, xylem vessel element differentiation is governed by the master regulators VASCULAR RELATED NAC-DOMAIN6 (VND6) and VND7, encoding plant-specific NAC transcription factors. Although vessel elements have not been found in bryophytes, differentiation of the water-conducting hydroid cells in the moss Physcomitrella patens is regulated by VND homologs termed VND-NST-SOMBRERO (VNS) genes. VNS genes are conserved in the land plant lineage, but their functions have not been elucidated outside of angiosperms and P. patens. The peat moss Sphagnum palustre, of class Sphagnopsida in the phylum Bryophyta, does not have hydroids and instead uses hyaline cells with thickened, helical-patterned cell walls and pores to store water in the leaves. Here, we performed whole-transcriptome analysis and de novo assembly using next generation sequencing in S. palustre, obtaining sequences for 68,305 genes. Among them, we identified seven VNS-like genes, SpVNS1-A, SpVNS1-B, SpVNS2-A, SpVNS2-B, SpVNS3-A, SpVNS3-B, and SpVNS4-A. Transient expression of these VNS-like genes, with the exception of SpVNS2-A, in Nicotiana benthamiana leaf cells resulted in ectopic thickening of secondary walls. This result suggests that the transcriptional activity observed in other VNS family members is functionally conserved in the VNS homologs of S. palustre.
Identifiants
pubmed: 33881701
doi: 10.1007/s11103-021-01148-6
pii: 10.1007/s11103-021-01148-6
doi:
Substances chimiques
Plant Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
309-317Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP20K06669
Organisme : Japan Society for the Promotion of Science
ID : JP25291062
Organisme : Japan Society for the Promotion of Science
ID : JP18H02466
Organisme : Nara Institute of Science and Technology
ID : Humanophilic Innovation Project
Organisme : Nara Institute of Science and Technology
ID : Big Data Project
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : JP24114002
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : JP18H05484
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : JP18H05489
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