Genome and transcriptome of a pathogenic yeast, Candida nivariensis.

Candida nivariensis genome assembly nanopore sequencing pathogenic yeast transcriptome

Journal

G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598

Informations de publication

Date de publication:
14 07 2021
Historique:
received: 05 02 2021
accepted: 11 04 2021
pubmed: 24 4 2021
medline: 15 11 2022
entrez: 23 4 2021
Statut: ppublish

Résumé

We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

Identifiants

pubmed: 33890630
pii: 6247763
doi: 10.1093/g3journal/jkab137
pmc: PMC8496292
pii:
doi:

Substances chimiques

RNA 63231-63-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NHGRI NIH HHS
ID : R01 HG009190
Pays : United States
Organisme : CIHR
ID : DFS-157831
Pays : Canada

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

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Auteurs

Yunfan Fan (Y)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Andrew N Gale (AN)

Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.

Anna Bailey (A)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Kali Barnes (K)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Kiersten Colotti (K)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Michal Mass (M)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Luke B Morina (LB)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Bailey Robertson (B)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Remy Schwab (R)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Niki Tselepidakis (N)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Winston Timp (W)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Division of Infectious Disease, Department of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.

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Classifications MeSH