MicroRNA Expression Differences in Blood-Derived CD19+ B Cells of Methotrexate Treated Rheumatoid Arthritis Patients.
Antigens, CD19
/ metabolism
Arthritis, Rheumatoid
/ drug therapy
B-Lymphocytes
/ drug effects
Biomarkers
Computational Biology
/ methods
Disease Management
Disease Susceptibility
Gene Expression Profiling
Gene Expression Regulation
/ drug effects
Gene Regulatory Networks
High-Throughput Nucleotide Sequencing
Humans
Immunosuppressive Agents
/ pharmacology
Methotrexate
/ pharmacology
MicroRNAs
/ genetics
RNA Interference
CD19+ B cells
NGS
methotrexate
miRNA
microRNA
rheumatoid arthritis
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
02
2021
accepted:
17
03
2021
entrez:
26
4
2021
pubmed:
27
4
2021
medline:
25
2
2023
Statut:
epublish
Résumé
Rheumatoid arthritis (RA) is a complex disease with a wide range of underlying susceptibility factors. Recently, dysregulation of microRNAs (miRNAs) in RA have been reported in several immune cell types from blood. However, B cells have not been studied in detail yet. Given the autoimmune nature of RA with the presence of autoantibodies, CD19+ B cells are a key cell type in RA pathogenesis and alterations in CD19+ B cell subpopulations have been observed in patient blood. Therefore, we aimed to reveal the global miRNA repertoire and to analyze miRNA expression profile differences in homogenous RA patient phenotypes in blood-derived CD19+ B cells. Small RNA sequencing was performed on CD19+ B cells of newly diagnosed untreated RA patients (n=10), successfully methotrexate (MTX) treated RA patients in remission (MTX treated RA patients, n=18) and healthy controls (n=9). The majority of miRNAs was detected across all phenotypes. However, significant expression differences between MTX treated RA patients and controls were observed for 27 miRNAs, while no significant differences were seen between the newly diagnosed patients and controls. Several of the differentially expressed miRNAs were previously found to be dysregulated in RA including miR-223-3p, miR-486-3p and miR-23a-3p. MiRNA target enrichment analysis, using the differentially expressed miRNAs and miRNA-target interactions from miRTarBase as input, revealed enriched target genes known to play important roles in B cell activation, differentiation and B cell receptor signaling, such as
Identifiants
pubmed: 33897713
doi: 10.3389/fimmu.2021.663736
pmc: PMC8062711
doi:
Substances chimiques
Antigens, CD19
0
Biomarkers
0
Immunosuppressive Agents
0
MicroRNAs
0
Methotrexate
YL5FZ2Y5U1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
663736Informations de copyright
Copyright © 2021 Heinicke, Zhong, Flåm, Breidenbach, Leithaug, Mæhlen, Lillegraven, Aga, Norli, Mjaavatten, Haavardsholm, Zucknick, Rayner and Lie.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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