First time β-farnesene production by the versatile bacterium Cupriavidus necator.
C. necator
Process design
Sustainable economy
Terpene production
β-farnesene
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
26 Apr 2021
26 Apr 2021
Historique:
received:
16
12
2020
accepted:
09
03
2021
entrez:
27
4
2021
pubmed:
28
4
2021
medline:
5
10
2021
Statut:
epublish
Résumé
Terpenes are remarkably diverse natural structures, which can be formed via two different pathways leading to two common intermediates. Among those, sesquiterpenes represent a variety of industrially relevant products. One important industrially produced product is β-farnesene as a precursor for a jet fuel additive. So far, microbial terpene production has been mostly limited to known production hosts, which are only able to grow on heterotrophic substrates. In this paper, we for the first time describe β-farnesene production by the versatile bacterial host Cupriavidus necator on fructose, which is known to grow hetero- and autotrophically and even in bioelectrochemical systems. We were able to show a growth-dependent production of β-farnesene by expressing the β-farnesene synthase from Artemisia annua in C. necator H16 PHB The β-farnesene production titers reported in this paper are not in the same range as titers published with known heterotrophic producers E. coli or S. cerevisiae. However, this proof-of-principle study with C. necator as production host opens new synthesis routes toward a sustainable economy and leaves room for further optimizations, which have been already performed with the known production strains.
Sections du résumé
BACKGROUND
BACKGROUND
Terpenes are remarkably diverse natural structures, which can be formed via two different pathways leading to two common intermediates. Among those, sesquiterpenes represent a variety of industrially relevant products. One important industrially produced product is β-farnesene as a precursor for a jet fuel additive. So far, microbial terpene production has been mostly limited to known production hosts, which are only able to grow on heterotrophic substrates.
RESULTS
RESULTS
In this paper, we for the first time describe β-farnesene production by the versatile bacterial host Cupriavidus necator on fructose, which is known to grow hetero- and autotrophically and even in bioelectrochemical systems. We were able to show a growth-dependent production of β-farnesene by expressing the β-farnesene synthase from Artemisia annua in C. necator H16 PHB
CONCLUSIONS
CONCLUSIONS
The β-farnesene production titers reported in this paper are not in the same range as titers published with known heterotrophic producers E. coli or S. cerevisiae. However, this proof-of-principle study with C. necator as production host opens new synthesis routes toward a sustainable economy and leaves room for further optimizations, which have been already performed with the known production strains.
Identifiants
pubmed: 33902586
doi: 10.1186/s12934-021-01562-x
pii: 10.1186/s12934-021-01562-x
pmc: PMC8074451
doi:
Substances chimiques
Sesquiterpenes
0
beta-farnesene
18794-84-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
89Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 031A226
Organisme : Bundesministerium für Bildung und Forschung
ID : 031B0347A
Références
J Microbiol Biotechnol. 2019 Oct 28;29(10):1656-1664
pubmed: 31546303
Biotechnol Adv. 2012 May-Jun;30(3):691-708
pubmed: 22244816
Appl Microbiol Biotechnol. 2019 Jul;103(14):5501-5516
pubmed: 31129740
J Sep Sci. 2013 Apr;36(8):1370-8
pubmed: 23404959
Cell Mol Life Sci. 2004 Jun;61(12):1401-26
pubmed: 15197467
Appl Environ Microbiol. 1976 Oct;32(4):585-91
pubmed: 984831
Arch Biochem Biophys. 2011 Jan 15;505(2):131-43
pubmed: 20932952
Biotechnol Bioeng. 2016 Jan;113(1):72-81
pubmed: 26108688
Appl Environ Microbiol. 1976 Oct;32(4):592-7
pubmed: 10840
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
J Agric Food Chem. 2019 Sep 4;67(35):9858-9867
pubmed: 31389230
Eng Life Sci. 2017 Apr 24;17(7):781-791
pubmed: 32624824
Nat Prod Rep. 2013 Sep;30(9):1226-64
pubmed: 23884176
Angew Chem Int Ed Engl. 2018 Feb 12;57(7):1879-1882
pubmed: 29232490
Annu Rev Microbiol. 1981;35:405-52
pubmed: 6271040
Materials (Basel). 2020 Mar 06;13(5):
pubmed: 32155730
Phytochemistry. 2005 May;66(9):961-7
pubmed: 15896363
J Mol Biol. 1983 Jun 5;166(4):557-80
pubmed: 6345791
ACS Synth Biol. 2018 Aug 17;7(8):1918-1928
pubmed: 29949349
Bioresour Technol. 2017 Nov;243:228-236
pubmed: 28672185