Noncanonical DNA polymerization by aminoadenine-based siphoviruses.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
30 04 2021
30 04 2021
Historique:
received:
04
09
2020
accepted:
25
03
2021
entrez:
30
4
2021
pubmed:
1
5
2021
medline:
14
5
2021
Statut:
ppublish
Résumé
Bacteriophage genomes harbor the broadest chemical diversity of nucleobases across all life forms. Certain DNA viruses that infect hosts as diverse as cyanobacteria, proteobacteria, and actinobacteria exhibit wholesale substitution of aminoadenine for adenine, thereby forming three hydrogen bonds with thymine and violating Watson-Crick pairing rules. Aminoadenine-encoded DNA polymerases, homologous to the Klenow fragment of bacterial DNA polymerase I that includes 3'-exonuclease but lacks 5'-exonuclease, were found to preferentially select for aminoadenine instead of adenine in deoxynucleoside triphosphate incorporation templated by thymine. Polymerase genes occur in synteny with genes for a biosynthesis enzyme that produces aminoadenine deoxynucleotides in a wide array of
Identifiants
pubmed: 33926956
pii: 372/6541/520
doi: 10.1126/science.abe6542
doi:
Substances chimiques
DNA, Viral
0
Viral Nonstructural Proteins
0
2-Aminopurine
452-06-2
2,6-diaminopurine
49P95BAU4Z
DNA-Directed DNA Polymerase
EC 2.7.7.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
520-524Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.