Engineering a ComA Quorum-Sensing circuit to dynamically control the production of Menaquinone-4 in Bacillus subtilis.

Bacillus subtilis / genetics Gene Expression Regulation, Bacterial Metabolic Engineering Quorum Sensing Vitamin K 2 / analogs & derivatives
Bacillus subtilis Dynamic regulation Menaquinone-4 PhrQ-RapQ-ComA

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Jun 2021
Historique:
received: 28 10 2020
revised: 09 03 2021
accepted: 11 03 2021
entrez: 16 5 2021
pubmed: 17 5 2021
medline: 19 8 2021
Statut: ppublish

Résumé

Menaquinone-4 (MK-4) plays a significant role in bone health and cardiovascular therapy. Although many strategies have been adopted to increase the yield of MK-4 in Bacillus subtilis 168, the effectiveness of MK-4 is still low due to the inherent limitations of metabolic pathways. However, dynamic regulation based on quorum sensing (QS) has been extensively applied as a fundamental tool for fine-tuning gene expression in reaction to changes in cell density without adding expensive inducers. Nevertheless, in most reports, QS systems depend on down-regulated expression rather than up-regulated expression, which greatly limit their potential as molecular switches to control metabolic flux. To address this challenge, a modular PhrQ-RapQ-ComA QS system is developed based on promoter P

Identifiants

DOI: 10.1016/j.enzmictec.2021.109782 PMID: 33992404
pubmed: 33992404
pii: S0141-0229(21)00040-5
doi: 10.1016/j.enzmictec.2021.109782
pii:
doi:

Substances chimiques

Vitamin K 2 11032-49-8
menatetrenone 27Y876D139

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109782

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Panhong Yuan (P)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Guoyun Sun (G)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Shixiu Cui (S)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Yaokang Wu (Y)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Xueqin Lv (X)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Yanfeng Liu (Y)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Jianghua Li (J)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Guocheng Du (G)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address: gcdu@jiangnan.edu.cn.

Long Liu (L)

Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address: longliu@jiangnan.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus subtilis › Engineering a ComA Quorum-Sensing circuit to...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Engineering a ComA Quorum-Sensing circuit to...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Engineering a ComA Quorum-Sensing circuit to...
questionsmedicales.fr › Phénomènes microbiologiques › Interactions microbiennes › Détection du quorum › Engineering a ComA Quorum-Sensing circuit to...
questionsmedicales.fr › Composés polycycliques › Hydrocarbures aromatiques polycycliques › Naphtalènes › Naphtoquinones › Vitamine K › Vitamine K2 › Engineering a ComA Quorum-Sensing circuit to...