Interlaboratory proficiency tests for the detection of staphylococcal enterotoxin type A in food.
Staphylococcus aureus
coagulase-positive staphylococci (CPS)
dairy products
food control
food safety
food-borne intoxication
immunoassay
staphylococcal food poisoning
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
26
04
2021
received:
23
12
2020
accepted:
12
05
2021
pubmed:
23
5
2021
medline:
15
9
2021
entrez:
22
5
2021
Statut:
ppublish
Résumé
Staphylococcal enterotoxins (SEs) are among the leading causes of food intoxications, affecting consumer health even in nanogram (ng) amounts. In the European Union, certain food safety criteria are specified, including the absence of SEs in cheeses, milk powder and whey powder. Until 2019, the analytical reference method used was the European Screening Method, which was replaced by EN ISO 19020. For the official laboratories involved in food control, the German Reference Laboratory for coagulase-positive staphylococci including Staphylococcus aureus organized three interlaboratory proficiency tests (ILPTs) to detect SE type A in food during the years 2013-2018. The selected food products (cream cheese and vanilla pudding) were successfully tested beforehand with regard to easy handling, homogeneity and stability of the added toxin. In 2013, ILPT participants overall were not competent in detecting SE type A in food. The following factors were identified to improve the performance: (i) concentration of sample extract using dialysis; (ii) selection of a sensitive detection kit; and (iii) proper sample handling. By taking these factors into account and instructing and training the laboratories, their competence greatly improved. In 2018, all performance criteria (specificity, sensitivity and accuracy) were >90%, even at very low concentrations of SE type A of approximately 0·01 ng g
Substances chimiques
Enterotoxins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
318-325Informations de copyright
© 2021 German Federal Institute for Risk Assessment and The French Agency for Food, Environmental and Occupational Health & Safety. Letters in Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.
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