Successful application of ancient DNA extraction and library construction protocols to museum wet collection specimens.
ancient DNA
archival DNA
biological collection
formalin
single-stranded DNA library
target capture
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
09
05
2021
received:
07
01
2021
accepted:
14
05
2021
pubmed:
27
5
2021
medline:
18
9
2021
entrez:
26
5
2021
Statut:
ppublish
Résumé
Millions of scientific specimens are housed in museum collections, a large part of which are fluid preserved. The use of formaldehyde as fixative and subsequent storage in ethanol is especially common in ichthyology and herpetology. This type of preservation damages DNA and reduces the chance of successful retrieval of genetic data. We applied ancient DNA extraction and single stranded library construction protocols to a variety of vertebrate samples obtained from wet collections and of different ages. Our results show that almost all samples tested yielded endogenous DNA. Archival DNA extraction was successful across different tissue types as well as using small amounts of tissue. Conversion of archival DNA fragments into single-stranded libraries resulted in usable data even for samples with initially undetectable DNA amounts. Subsequent target capture approaches for mitochondrial DNA using homemade baits on a subset of 30 samples resulted in almost complete mitochondrial genome sequences in several instances. Thus, application of ancient DNA methodology makes wet collection specimens, including type material as well as rare, old or extinct species, accessible for genetic and genomic analyses. Our results, accompanied by detailed step-by-step protocols, are a large step forward to open the DNA archive of museum wet collections for scientific studies.
Identifiants
pubmed: 34036732
doi: 10.1111/1755-0998.13433
doi:
Substances chimiques
DNA, Ancient
0
DNA, Mitochondrial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2299-2315Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : #2013/50741-7
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : #2017/2616-8
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : #2018/15425-0
Organisme : Deutsche Forschungsgemeinschaft
ID : 351649567
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 431589/2016-0
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 306623/2018-8
Informations de copyright
© 2021 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
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