The splicing factor XAB2 interacts with ERCC1-XPF and XPG for R-loop processing.
Animals
Cell Line
DNA Damage
/ drug effects
DNA Repair
DNA-Binding Proteins
/ metabolism
Endonucleases
/ metabolism
Female
Gene Expression Regulation, Developmental
Gene Knock-In Techniques
Gene Knockdown Techniques
Liver
/ growth & development
Male
Mice
Mice, Transgenic
Mouse Embryonic Stem Cells
Nuclear Proteins
/ metabolism
Polycyclic Sesquiterpenes
/ pharmacology
R-Loop Structures
/ genetics
RNA Precursors
/ genetics
RNA Splicing Factors
/ genetics
RNA, Small Nuclear
RNA-Seq
Recombinant Proteins
/ genetics
Spliceosomes
/ metabolism
Transcription Factors
/ metabolism
Transcription, Genetic
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 05 2021
26 05 2021
Historique:
received:
20
07
2020
accepted:
27
04
2021
entrez:
27
5
2021
pubmed:
28
5
2021
medline:
10
6
2021
Statut:
epublish
Résumé
RNA splicing, transcription and the DNA damage response are intriguingly linked in mammals but the underlying mechanisms remain poorly understood. Using an in vivo biotinylation tagging approach in mice, we show that the splicing factor XAB2 interacts with the core spliceosome and that it binds to spliceosomal U4 and U6 snRNAs and pre-mRNAs in developing livers. XAB2 depletion leads to aberrant intron retention, R-loop formation and DNA damage in cells. Studies in illudin S-treated cells and Csb
Identifiants
pubmed: 34039990
doi: 10.1038/s41467-021-23505-1
pii: 10.1038/s41467-021-23505-1
pmc: PMC8155215
doi:
Substances chimiques
DNA excision repair protein ERCC-5
0
DNA-Binding Proteins
0
Nuclear Proteins
0
Polycyclic Sesquiterpenes
0
RNA Precursors
0
RNA Splicing Factors
0
RNA, Small Nuclear
0
Recombinant Proteins
0
Transcription Factors
0
U4 small nuclear RNA
0
U6 small nuclear RNA
0
XAB2 protein, mouse
0
xeroderma pigmentosum group F protein
0
Endonucleases
EC 3.1.-
Ercc1 protein, mouse
EC 3.1.-
illudin S
EWJ0GY2H7X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3153Références
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14602-7
pubmed: 9405659
Mol Cell. 2003 Jul;12(1):5-14
pubmed: 12887888
Curr Protoc Mol Biol. 2017 Oct 2;120:4.29.1-4.29.18
pubmed: 28967997
Gene. 2004 Mar 17;328:1-16
pubmed: 15019979
Science. 1992 Nov 27;258(5087):1463-6
pubmed: 1279808
Cell. 1997 May 2;89(3):425-35
pubmed: 9150142
Nat Commun. 2018 Oct 15;9(1):4281
pubmed: 30323192
Mol Vis. 2006 Oct 26;12:1259-71
pubmed: 17110909
Mol Cell. 2006 Sep 1;23(5):673-84
pubmed: 16949364
Cell Rep. 2018 May 8;23(6):1891-1905
pubmed: 29742442
Science. 2012 Dec 21;338(6114):1587-93
pubmed: 23258890
Cell Death Dis. 2016 Oct 13;7(10):e2409
pubmed: 27735937
Adv Exp Med Biol. 2007;623:190-211
pubmed: 18380348
Genes Dev. 2015 Jan 1;29(1):63-80
pubmed: 25561496
Nat Genet. 2002 Apr;30(4):377-84
pubmed: 11925564
Genome Res. 2014 Nov;24(11):1774-86
pubmed: 25258385
Mol Cell. 2011 Jun 24;42(6):794-805
pubmed: 21700224
Nat Struct Mol Biol. 2010 Nov;17(11):1358-66
pubmed: 20972445
EMBO J. 2005 Nov 16;24(22):3952-62
pubmed: 16252008
Front Genet. 2016 Oct 25;7:187
pubmed: 27826317
Sci Rep. 2018 Mar 1;8(1):3850
pubmed: 29497062
Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):E10437-E10446
pubmed: 30322915
Mol Cell Biol. 2006 Aug;26(15):5744-58
pubmed: 16847328
Genetics. 2000 Dec;156(4):1503-17
pubmed: 11102353
Future Oncol. 2006 Apr;2(2):191-9
pubmed: 16563088
Elife. 2016 Aug 23;5:
pubmed: 27552054
Trends Genet. 2012 Nov;28(11):566-73
pubmed: 22824526
Electrophoresis. 2004 May;25(9):1327-33
pubmed: 15174055
J Biol Chem. 2008 Jan 11;283(2):940-50
pubmed: 17981804
PLoS One. 2010 Jul 21;5(7):e11695
pubmed: 20657786
Mol Cell. 2003 Sep;12(3):711-21
pubmed: 14527416
Biochem Soc Trans. 2010 Oct;38(5):1251-6
pubmed: 20863294
Biomolecules. 2015 Oct 29;5(4):2935-77
pubmed: 26529031
Mol Cell. 2014 Dec 18;56(6):777-85
pubmed: 25435140
Mol Cell Biol. 2006 Apr;26(7):2531-9
pubmed: 16537899
Cell. 2007 Jun 29;129(7):1311-23
pubmed: 17604720
Hum Mol Genet. 2011 Dec 1;20(23):4540-55
pubmed: 21875900
PLoS Genet. 2008 Aug 15;4(8):e1000161
pubmed: 18704162
Genes Dev. 2011 Jun 1;25(11):1147-58
pubmed: 21576257
Cold Spring Harb Perspect Biol. 2013 Oct 01;5(10):a012609
pubmed: 24086042
Nature. 1996 Feb 1;379(6564):466-9
pubmed: 8559255
N Engl J Med. 2009 Oct 8;361(15):1475-85
pubmed: 19812404
Nucleic Acids Res. 2018 Nov 30;46(21):11357-11369
pubmed: 30357359
Genes Dev. 2010 Jul 1;24(13):1434-47
pubmed: 20595234
Nat Cell Biol. 2017 May;19(5):421-432
pubmed: 28368372
Genome Res. 2017 Oct;27(10):1759-1768
pubmed: 28855263
Cell Metab. 2013 Sep 3;18(3):403-15
pubmed: 24011075
Bioessays. 2019 Apr;41(4):e1800201
pubmed: 30919497
Trends Genet. 2008 Feb;24(2):77-85
pubmed: 18192065
J Biol Chem. 2005 Nov 18;280(46):38862-9
pubmed: 16162498
Cell Rep. 2019 Sep 17;28(12):3167-3181.e6
pubmed: 31533039
Nat Genet. 2012 May;44(5):598-602
pubmed: 22466611
Genes Dev. 2016 Jun 1;30(11):1327-38
pubmed: 27298336
Mol Cell. 2011 Aug 5;43(3):353-68
pubmed: 21816343
Nature. 2015 Jul 2;523(7558):53-8
pubmed: 26106861
Nat Cell Biol. 2008 Nov;10(11):1241-7
pubmed: 18978832
Int J Cell Biol. 2013;2013:153634
pubmed: 24159334
Front Genet. 2013 Jun 06;4:102
pubmed: 23761808
Nat Genet. 2011 Dec 11;44(1):47-52
pubmed: 22158541
Nat Commun. 2013;4:1336
pubmed: 23299886
Nucleic Acids Res. 2014 Jan;42(2):701-13
pubmed: 24081581
Nucleic Acids Res. 2016 Jul 8;44(12):5702-16
pubmed: 27084940
Trends Genet. 2020 Oct;36(10):777-791
pubmed: 32684438
Mol Cell. 2003 Aug;12(2):525-32
pubmed: 14536091
Nucleic Acids Res. 2019 Sep 5;47(15):8239-8254
pubmed: 31216022
Nature. 1998 Jun 18;393(6686):702-5
pubmed: 9641683
Cell. 2005 Aug 12;122(3):365-78
pubmed: 16096057
Int J Mol Sci. 2018 Feb 23;19(2):
pubmed: 29473878
Nat Protoc. 2009;4(5):698-705
pubmed: 19373234
Mol Cell Biol. 2010 May;30(9):2105-19
pubmed: 20176811
Curr Opin Cell Biol. 2005 Jun;17(3):251-6
pubmed: 15901493
Mol Cell. 2014 Jan 23;53(2):235-246
pubmed: 24332808
Mech Ageing Dev. 2007 Jan;128(1):58-63
pubmed: 17126380
Cell. 2009 May 15;137(4):708-20
pubmed: 19450518
Trends Biochem Sci. 2012 May;37(5):179-88
pubmed: 22480731
Trends Pharmacol Sci. 2006 Jun;27(6):338-44
pubmed: 16697054
Structure. 2012 Mar 7;20(3):397-405
pubmed: 22404999
DNA Repair (Amst). 2002 Dec 5;1(12):1027-38
pubmed: 12531012
Cell. 2014 Dec 18;159(7):1511-23
pubmed: 25525873
DNA Repair (Amst). 2005 Apr 4;4(4):479-91
pubmed: 15725628
Cell. 2017 Aug 10;170(4):774-786.e19
pubmed: 28802045
Nucleic Acids Res. 2010 May;38(9):3005-18
pubmed: 20110258
J Biol Chem. 2000 Nov 10;275(45):34931-7
pubmed: 10944529
RNA. 2004 Oct;10(10):1489-98
pubmed: 15383674
Nat Struct Mol Biol. 2006 Nov;13(11):973-80
pubmed: 17028590
Nat Commun. 2020 Jan 2;11(1):42
pubmed: 31896748
Science. 2003 Oct 10;302(5643):279-82
pubmed: 12970570
Methods Mol Biol. 2005;291:303-20
pubmed: 15502232