Novel extraction chemistry and alternative amplification strategies for use with rootless hair shafts.
DNA analysis/testing
bi-allelic markers
capillary electrophoresis
degraded DNA
next-generation sequencing
rootless hair shaft
Journal
Journal of forensic sciences
ISSN: 1556-4029
Titre abrégé: J Forensic Sci
Pays: United States
ID NLM: 0375370
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
14
04
2021
received:
05
03
2021
accepted:
06
05
2021
pubmed:
1
6
2021
medline:
8
9
2021
entrez:
31
5
2021
Statut:
ppublish
Résumé
Rootless hair shafts are often considered unsuitable for STR genotyping due to the known high failure rate. The same samples can be reliably processed with mitochondrial sequencing. However, the minimal discriminatory power of widely implemented control region mitochondrial sequencing techniques limits its utility in some forensic casework. In this research, multiple variables were tested to provide information on rootless hair shaft sample genotyping success. Results showed external decontamination procedures decreased drop-in alleles but also greatly reduced profile recovery. The novel InnoXtract
Identifiants
pubmed: 34057738
doi: 10.1111/1556-4029.14763
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1929-1936Subventions
Organisme : Lucas Foundation
Informations de copyright
© 2021 American Academy of Forensic Sciences.
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