Morphological and molecular characterization of Neopestalotiopsis vitis associated with leaf blight disease of Manilkara zapota-a new record from India.
Neopestalotiopsis vitis
detached leaf assay
disease incidence
leaf blight
molecular taxonomy
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
01
06
2021
received:
12
04
2021
accepted:
02
06
2021
pubmed:
8
6
2021
medline:
15
9
2021
entrez:
7
6
2021
Statut:
ppublish
Résumé
Sapota is an important horticultural crop grown in India, and Karnataka is a major producer of sapota. A characteristic leaf blight disease was observed in Southern Karnataka during field surveys conducted in 2019 with an incidence of 13-22% in approximately 45 ha of sapota field. The leaf blight-associated pathogen was isolated on the potato dextrose agar medium. A total of 12 isolates obtained from each location were identified culturally and morphologically. Based on the morphological and cultural features, the pathogen was identified as Pestalotiopsis or Neopestalotiopsis, which was further confirmed by molecular identification using a representative isolate (MZ03). The ITS rDNA and β-tubulin genes were amplified and sequenced using ITS1/ITS4 and T1/T22 primer pairs respectively. nBLAST search analysis and concatenated (ITS-rDNA and TUB2 loci) phylogenetic analysis confirmed the pathogen identity as Neopestalotiopsis vitis. Pathogenicity tests conducted on detached leaves by inoculation with a conidial suspension of N. vitis produced typical blight symptoms after 4-5 days and progressed to cover the entire leaf lamina after 10-12 days. The pathogen's identity was confirmed after re-isolation by cultural and morphological features. Although Pestalotiopsis clavispora and Pestalotiopsis versicolor causing diseases on sapota seedlings and trees have been reported, no reports are available for the occurrence of N. vitis to sapota from India. This is the first report of N. vitis associated with leaf blight disease of sapota from India.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
352-362Informations de copyright
© 2021 The Society for Applied Microbiology.
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