Anchor extension: a structure-guided approach to design cyclic peptides targeting enzyme active sites.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
07 06 2021
Historique:
received: 09 06 2020
accepted: 04 05 2021
entrez: 8 6 2021
pubmed: 9 6 2021
medline: 16 6 2021
Statut: epublish

Résumé

Despite recent success in computational design of structured cyclic peptides, de novo design of cyclic peptides that bind to any protein functional site remains difficult. To address this challenge, we develop a computational "anchor extension" methodology for targeting protein interfaces by extending a peptide chain around a non-canonical amino acid residue anchor. To test our approach using a well characterized model system, we design cyclic peptides that inhibit histone deacetylases 2 and 6 (HDAC2 and HDAC6) with enhanced potency compared to the original anchor (IC

Identifiants

pubmed: 34099674
doi: 10.1038/s41467-021-23609-8
pii: 10.1038/s41467-021-23609-8
pmc: PMC8185074
doi:

Substances chimiques

Histone Deacetylase Inhibitors 0
Peptide Library 0
Peptides, Cyclic 0
Recombinant Proteins 0
Zebrafish Proteins 0
HDAC2 protein, human EC 3.5.1.98
HDAC6 protein, human EC 3.5.1.98
HDAC6 protein, zebrafish EC 3.5.1.98
Histone Deacetylase 2 EC 3.5.1.98
Histone Deacetylase 6 EC 3.5.1.98

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

3384

Subventions

Organisme : NIGMS NIH HHS
ID : F32 GM120791
Pays : United States
Organisme : NIA NIH HHS
ID : P50 AG005136
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM049758
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122543
Pays : United States

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Auteurs

Parisa Hosseinzadeh (P)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.
Knight Campus Center, University of Oregon, Eugene, OR, USA.

Paris R Watson (PR)

Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA.

Timothy W Craven (TW)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.

Xinting Li (X)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.

Stephen Rettie (S)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.
Molecular and Cellular Biology Ph.D. Program, University of Washington, Seattle, WA, USA.

Fátima Pardo-Avila (F)

Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA.

Asim K Bera (AK)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.

Vikram Khipple Mulligan (VK)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.
Systems Biology, Center for Computational Biology, Flatiron Institute, New York, NY, USA.

Peilong Lu (P)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.
Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.

Alexander S Ford (AS)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.

Brian D Weitzner (BD)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.
Lyell Immunopharma, Inc., Seattle, WA, USA.

Lance J Stewart (LJ)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.

Adam P Moyer (AP)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.
Molecular Engineering Ph.D. Program, University of Washington, Seattle, WA, USA.

Maddalena Di Piazza (M)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.

Joshua G Whalen (JG)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.

Per Jr Greisen (PJ)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA.
Novo Nordisk A/S, Måløv, Denmark.

David W Christianson (DW)

Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA.

David Baker (D)

University of Washington, Department of Biochemistry, Institute for Protein Design, Seattle, WA, USA. dabaker@uw.edu.

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Classifications MeSH