The emerging landscape of single-molecule protein sequencing technologies.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
04
06
2020
accepted:
02
04
2021
entrez:
8
6
2021
pubmed:
9
6
2021
medline:
28
8
2021
Statut:
ppublish
Résumé
Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.
Identifiants
pubmed: 34099939
doi: 10.1038/s41592-021-01143-1
pii: 10.1038/s41592-021-01143-1
pmc: PMC8223677
mid: NIHMS1716427
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
604-617Subventions
Organisme : NCI NIH HHS
ID : UH3 CA246635
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM108569
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG011087
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP1 GM133052
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122480
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM138931
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG009186
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB018659
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK110520
Pays : United States
Organisme : NIH HHS
ID : DP2 OD007292
Pays : United States
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