RNA Sequencing for Transcript 5'-End Mapping in Mycobacteria.
Endoribonuclease
Mycobacterium smegmatis
Mycobacterium tuberculosis
RNA 5′ ends
RNA processing
RNA sequencing
RNA-seq
RNase
RNase cleavage
Transcription start site
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
8
7
2021
pubmed:
9
7
2021
medline:
7
8
2021
Statut:
ppublish
Résumé
Next-generation sequencing technologies facilitate the analysis of multiple important properties of transcriptomes in addition to gene expression levels. Here, we describe a method for mapping RNA 5' ends in Mycobacterium tuberculosis and Mycobacterium smegmatis, which allows the determination of transcription start sites (TSSs), comparative analysis of promoter usage under different conditions, and mapping of endoribonucleolytic cleavage sites. We describe in detail the procedures for constructing RNA sequencing libraries appropriate for RNA 5' end mapping using an Illumina sequencing platform, as well as bioinformatic procedures for data analysis.
Identifiants
pubmed: 34235668
doi: 10.1007/978-1-0716-1460-0_22
doi:
Substances chimiques
5' Untranslated Regions
0
RNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
513-531Références
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