Molecular topography of an entire nervous system.
Animals
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ genetics
Fluorescent Dyes
/ metabolism
Gene Expression Regulation, Developmental
Genes, Reporter
Larva
/ metabolism
Nervous System
/ metabolism
Neurons
/ metabolism
Neuropeptides
/ genetics
Nucleotide Motifs
/ genetics
RNA-Seq
Regulatory Sequences, Nucleic Acid
/ genetics
Signal Transduction
/ genetics
Transcription Factors
/ metabolism
Transcription, Genetic
C. elegans
RNA-seq
cell adhesion molecules
connectome
gene regulatory motifs
neuron
neuropeptides
single-cell
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
05 08 2021
05 08 2021
Historique:
received:
14
12
2020
revised:
09
04
2021
accepted:
14
06
2021
pubmed:
9
7
2021
medline:
6
1
2022
entrez:
8
7
2021
Statut:
ppublish
Résumé
We have produced gene expression profiles of all 302 neurons of the C. elegans nervous system that match the single-cell resolution of its anatomy and wiring diagram. Our results suggest that individual neuron classes can be solely identified by combinatorial expression of specific gene families. For example, each neuron class expresses distinct codes of ∼23 neuropeptide genes and ∼36 neuropeptide receptors, delineating a complex and expansive "wireless" signaling network. To demonstrate the utility of this comprehensive gene expression catalog, we used computational approaches to (1) identify cis-regulatory elements for neuron-specific gene expression and (2) reveal adhesion proteins with potential roles in process placement and synaptic specificity. Our expression data are available at https://cengen.org and can be interrogated at the web application CengenApp. We expect that this neuron-specific directory of gene expression will spur investigations of underlying mechanisms that define anatomy, connectivity, and function throughout the C. elegans nervous system.
Identifiants
pubmed: 34237253
pii: S0092-8674(21)00758-3
doi: 10.1016/j.cell.2021.06.023
pmc: PMC8710130
mid: NIHMS1716840
pii:
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Fluorescent Dyes
0
Neuropeptides
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4329-4347.e23Subventions
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Organisme : NICHD NIH HHS
ID : T32 HD007149
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK058404
Pays : United States
Organisme : NCRR NIH HHS
ID : G20 RR030956
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS100547
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK020593
Pays : United States
Organisme : NCRR NIH HHS
ID : UL1 RR024975
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK059637
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY008126
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007328
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA068485
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS110391
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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