Cadaverine regulates biotin synthesis to modulate primary root growth in Arabidopsis.
Acetyl-CoA Carboxylase
/ genetics
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Biotin
/ biosynthesis
Biotinylation
Cadaverine
/ metabolism
Carbon-Nitrogen Ligases
/ genetics
Fatty Acid Synthase, Type II
/ genetics
Fatty Acids
/ metabolism
Gene Expression Regulation, Plant
Mutation
Phenotype
Plant Roots
/ genetics
Seedlings
/ genetics
Transaminases
/ genetics
Arabidopsis thaliana
biotin
cadaverine
polyamines
root architecture
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
23
06
2021
received:
17
03
2021
accepted:
06
07
2021
pubmed:
13
7
2021
medline:
27
1
2022
entrez:
12
7
2021
Statut:
ppublish
Résumé
Cadaverine, a polyamine, has been linked to modification of root growth architecture and response to environmental stresses in plants. However, the molecular mechanisms that govern the regulation of root growth by cadaverine are largely unexplored. Here we conducted a forward genetic screen and isolated a mutation, cadaverine hypersensitive 3 (cdh3), which resulted in increased root-growth sensitivity to cadaverine, but not other polyamines. This mutation affects the BIO3-BIO1 biotin biosynthesis gene. Exogenous supply of biotin and a pathway intermediate downstream of BIO1, 7,8-diaminopelargonic acid, suppressed this cadaverine sensitivity phenotype. An in vitro enzyme assay showed cadaverine inhibits the BIO3-BIO1 activity. Furthermore, cadaverine-treated seedlings displayed reduced biotinylation of Biotin Carboxyl Carrier Protein 1 of the acetyl-coenzyme A carboxylase complex involved in de novo fatty acid biosynthesis, resulting in decreased accumulation of triacylglycerides. Taken together, these results revealed an unexpected role of cadaverine in the regulation of biotin biosynthesis, which leads to modulation of primary root growth of plants.
Identifiants
pubmed: 34250670
doi: 10.1111/tpj.15417
pmc: PMC8518694
doi:
Substances chimiques
Arabidopsis Proteins
0
BIO3-BIO1 fusion protein, Arabidopsis
0
Fatty Acids
0
Biotin
6SO6U10H04
Transaminases
EC 2.6.1.-
Fatty Acid Synthase, Type II
EC 6.-
Carbon-Nitrogen Ligases
EC 6.3.-
Acetyl-CoA Carboxylase
EC 6.4.1.2
biotin carboxyl carrier protein
EC 6.4.1.2
Cadaverine
L90BEN6OLL
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1283-1298Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103418
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR013790
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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