Molecular mechanism for kinesin-1 direct membrane recognition.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
20
01
2021
accepted:
11
06
2021
entrez:
29
7
2021
pubmed:
30
7
2021
medline:
16
4
2022
Statut:
epublish
Résumé
The cargo-binding capabilities of cytoskeletal motor proteins have expanded during evolution through both gene duplication and alternative splicing. For the light chains of the kinesin-1 family of microtubule motors, this has resulted in an array of carboxyl-terminal domain sequences of unknown molecular function. Here, combining phylogenetic analyses with biophysical, biochemical, and cell biology approaches, we identify a highly conserved membrane-induced curvature-sensitive amphipathic helix within this region of a subset of long kinesin light-chain paralogs and splice isoforms. This helix mediates the direct binding of kinesin-1 to lipid membranes. Membrane binding requires specific anionic phospholipids, and it contributes to kinesin-1-dependent lysosome positioning, a canonical activity that, until now, has been attributed exclusively the recognition of organelle-associated cargo adaptor proteins. This leads us to propose a protein-lipid coincidence detection framework for kinesin-1-mediated organelle transport.
Identifiants
pubmed: 34321209
pii: 7/31/eabg6636
doi: 10.1126/sciadv.abg6636
pmc: PMC8318374
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Lipids
0
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L01386X/1
Pays : United Kingdom
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
Références
Curr Biol. 2021 Feb 8;31(3):540-554.e5
pubmed: 33232665
Biochemistry. 2008 Apr 15;47(15):4535-43
pubmed: 18361505
Biochemistry. 2002 Apr 30;41(17):5566-72
pubmed: 11969417
Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12151-6
pubmed: 20566873
J Neurosci. 2000 Sep 1;20(17):6374-84
pubmed: 10964943
Mol Biol Cell. 2015 Apr 1;26(7):1296-307
pubmed: 25657321
Mol Biol Evol. 2020 May 1;37(5):1530-1534
pubmed: 32011700
DNA Cell Biol. 1993 Dec;12(10):881-92
pubmed: 8274221
Dev Cell. 2016 May 23;37(4):326-336
pubmed: 27219061
Proteins. 2005 Jun 1;59(4):687-96
pubmed: 15815974
Science. 2011 Apr 8;332(6026):247-51
pubmed: 21436401
Bioinformatics. 2008 Sep 15;24(18):2101-2
pubmed: 18662927
Cell Chem Biol. 2021 Mar 31;:
pubmed: 33838110
Genomics. 1996 Jun 15;34(3):399-409
pubmed: 8786141
Dev Cell. 2011 Dec 13;21(6):1171-8
pubmed: 22172677
Nat Cell Biol. 2000 Jun;2(6):333-8
pubmed: 10854323
Nat Cell Biol. 2016 Apr;18(4):404-17
pubmed: 26950892
Nature. 2002 May 2;417(6884):83-7
pubmed: 11986669
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Science. 2000 Sep 22;289(5487):2120-2
pubmed: 11000113
DNA Cell Biol. 1993 Dec;12(10):901-9
pubmed: 8274223
J Cell Biol. 2001 Mar 5;152(5):959-70
pubmed: 11238452
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W244-8
pubmed: 15980461
J Cell Sci. 2013 May 1;126(Pt 9):2027-41
pubmed: 23487040
Hum Mol Genet. 2015 Dec 15;24(24):6877-85
pubmed: 26385635
J Cell Biol. 1991 May;113(4):817-33
pubmed: 1827446
Genomics. 1998 Sep 1;52(2):209-13
pubmed: 9782088
Neuron. 1994 May;12(5):1059-72
pubmed: 7514426
Nat Rev Mol Cell Biol. 2009 Oct;10(10):682-96
pubmed: 19773780
J Cell Sci. 2016 Dec 1;129(23):4329-4339
pubmed: 27799357
ACS Synth Biol. 2012 Jun 15;1(6):240-50
pubmed: 23651206
EMBO J. 2006 Nov 29;25(23):5457-68
pubmed: 17093494
Nat Rev Mol Cell Biol. 2009 Nov;10(11):765-77
pubmed: 19851335
Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):2418-23
pubmed: 26884162
J Hum Genet. 2015 Dec;60(12):763-8
pubmed: 26423925
J Cell Biol. 2021 Jan 4;220(1):
pubmed: 33284322
Structure. 2018 Nov 6;26(11):1486-1498.e6
pubmed: 30197037
J Biomol NMR. 1995 Nov;6(3):277-93
pubmed: 8520220
Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2638-43
pubmed: 24497505
Biochemistry (Mosc). 2017 Jul;82(7):803-815
pubmed: 28918744
J Cell Biol. 2007 Jan 1;176(1):11-7
pubmed: 17200414
J Cell Biol. 1992 Jun;117(6):1263-75
pubmed: 1607388
Nat Rev Neurol. 2019 Dec;15(12):691-703
pubmed: 31558780
J Cell Sci. 2000 Jun;113 ( Pt 11):2047-54
pubmed: 10806115
Nucleic Acids Res. 2015 Jul 1;43(W1):W389-94
pubmed: 25883141
Curr Opin Cell Biol. 2019 Aug;59:16-23
pubmed: 30952037
EMBO J. 2011 Aug 31;30(17):3527-39
pubmed: 21878994
Neurosci Lett. 2015 Sep 25;605:18-23
pubmed: 26232680
Science. 2013 Apr 19;340(6130):356-9
pubmed: 23519214
EMBO J. 2002 Feb 1;21(3):281-93
pubmed: 11823421
J Biol Chem. 1994 Jan 14;269(2):1477-85
pubmed: 8288613
Dev Cell. 2015 Apr 20;33(2):176-88
pubmed: 25898167
BMC Evol Biol. 2010 Apr 27;10:110
pubmed: 20423470
J Mol Biol. 1994 Jul 29;240(5):507-12
pubmed: 8046755
Elife. 2018 Oct 15;7:
pubmed: 30320553
J Mol Biol. 1993 May 5;231(1):155-8
pubmed: 8496962
Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10114-8
pubmed: 1946431
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9654-9
pubmed: 9275178
Cell. 2003 Feb 21;112(4):467-80
pubmed: 12600311
Mol Biol Cell. 1998 Feb;9(2):333-43
pubmed: 9450959
Biochemistry. 2004 Oct 26;43(42):13525-31
pubmed: 15491159
Traffic. 2003 Aug;4(8):576-80
pubmed: 12839500
Trends Cell Biol. 2017 Jul;27(7):505-514
pubmed: 28284467
Biopolymers. 1991 Nov;31(13):1463-70
pubmed: 1814498
Cell. 1985 Aug;42(1):39-50
pubmed: 3926325
J Cell Sci. 2017 May 1;130(9):1637-1651
pubmed: 28302907
J Biol Chem. 1993 Jun 25;268(18):13657-66
pubmed: 8514798
EMBO J. 2011 Nov 16;30(22):4523-38
pubmed: 21915095
J Biol Chem. 1998 Jun 19;273(25):15395-403
pubmed: 9624122
Dev Cell. 2017 Oct 23;43(2):186-197.e7
pubmed: 29033361
Nucleic Acids Res. 2008 Apr;36(7):2295-300
pubmed: 18287115