The interactions of bacteriophage Ace and Shiga toxin-producing Escherichia coli during biocontrol.
Shiga Toxin-producing Escherichia coli
bacteriophage insensitive mutants
bacteriophages
biocontrol
environmental persistence
human safety
Journal
FEMS microbiology ecology
ISSN: 1574-6941
Titre abrégé: FEMS Microbiol Ecol
Pays: England
ID NLM: 8901229
Informations de publication
Date de publication:
03 08 2021
03 08 2021
Historique:
received:
19
02
2021
accepted:
28
07
2021
pubmed:
31
7
2021
medline:
11
8
2021
entrez:
30
7
2021
Statut:
ppublish
Résumé
Strictly lytic phages are considered powerful tools for biocontrol of foodborne pathogens. Safety issues needed to be addressed for the biocontrol of Shiga toxin-producing Escherichia coli (STEC) include: lysogenic conversion, Shiga toxin production through phage induction, and emergence/proliferation of bacteriophage insensitive mutants (BIMs). To address these issues, two new lytic phages, vB_EcoS_Ace (Ace) and vB_EcoM_Shy (Shy), were isolated and characterized for life cycle, genome sequence and annotation, pH stability and efficacy at controlling STEC growth. Ace was efficient in controlling host planktonic cells and did not stimulate the production of the Stx prophage or Shiga toxin. A single dose of phage did not lead to the selection of BIMs. However, when reintroduced, BIMs were detected after 24 h of incubation. The gain of resistance was associated with lower virulence, as a subset of BIMs failed to agglutinate with O157-specific antibody and were more sensitive to human serum complement. BIM's biofilm formation capacity and susceptibility to disinfectants was equal to that of the wild-type strain. Overall, this work demonstrated that phage Ace is a safe biocontrol agent against STEC contamination and that the burden of BIM emergence did not represent a greater risk in environmental persistence and human pathogenicity.
Identifiants
pubmed: 34329454
pii: 6332279
doi: 10.1093/femsec/fiab105
pmc: PMC8492476
pii:
doi:
Substances chimiques
Biological Control Agents
0
Shiga Toxin 2
0
Shiga Toxin
75757-64-1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103408
Pays : United States
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
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