Genomic insights into the antibiotic resistance pattern of the tetracycline-degrading bacterium, Arthrobacter nicotianae OTC-16.

Biodegradation, Environmental Drug Resistance, Microbial Genomics Micrococcaceae Oxytetracycline Protein Synthesis Inhibitors

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
02 08 2021
Historique:
received: 01 04 2021
accepted: 14 07 2021
entrez: 3 8 2021
pubmed: 4 8 2021
medline: 6 11 2021
Statut: epublish

Résumé

Although many bacteria have the potential to remove antibiotic residues from environmental niches, the benefits of using antibiotic-degrading bacteria to manage antibiotic pollution should be assessed against the risk of the potential expansion of antimicrobial resistance. This study investigated the antibiotic resistance pattern of the bacterium Arthrobacter nicotianae OTC-16, which shows substantial biodegradation of oxytetracycline (OTC)/tetracycline. The results showed that this strain could be resistant to at least seven categories of 15 antibiotics, based on antimicrobial susceptibility testing. The genome of A. nicotianae OTC-16 contains one chromosome (3,643,989 bp) and two plasmids (plasmid1, 123,894 bp and plasmid2, 29,841 bp). Of the 3,561 genes isolated, eight were related to antibiotic resistance. During OTC degradation by the strain OTC-16, the expression of ant2ia, sul1, tet33, and cml_e8 in the plasmid, and one gene (tetV) in the chromosome were tracked using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Only the plasmid-derived resistance genes were up-regulated in the presence of OTC. The presence of OTC increased the tolerance of strain OTC-16 to streptomycin sulphate. The findings of this study can help deepen our understanding of the behavioural characteristics of resistance genes and adaptive evolution of drug-resistant bacteria.

Identifiants

DOI: 10.1038/s41598-021-94840-y PMID: 34341372 PMC: PMC8329189
pubmed: 34341372
doi: 10.1038/s41598-021-94840-y
pii: 10.1038/s41598-021-94840-y
pmc: PMC8329189
doi:

Substances chimiques

Protein Synthesis Inhibitors 0
Oxytetracycline X20I9EN955

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15638

Informations de copyright

© 2021. The Author(s).

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Auteurs

Xin Zhang (X)

College of Forest and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China. zhangxins@126.com.

Rongrong Zhu (R)

College of Forest and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China.
The Institute of Environment, Resources, Soil and Fertilizers, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Weilin Li (W)

College of Forest and Biotechnology, Zhejiang A & F University, Hangzhou, 311300, China.
The Institute of Environment, Resources, Soil and Fertilizers, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Junwei Ma (J)

The Institute of Environment, Resources, Soil and Fertilizers, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Hui Lin (H)

The Institute of Environment, Resources, Soil and Fertilizers, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. linhui@zaas.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Santé publique › Pollution de l'environnement › Assainissement et restauration de l'environnement › Dépollution biologique de l'environnement › Genomic insights into the antibiotic...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Genomic insights into the antibiotic...
questionsmedicales.fr › Disciplines des sciences naturelles › Disciplines des sciences biologiques › Biologie › Génétique › Génomique › Genomic insights into the antibiotic...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Micrococcaceae › Genomic insights into the antibiotic...
questionsmedicales.fr › Composés polycycliques › Hydrocarbures aromatiques polycycliques › Naphtacènes › Tétracyclines › Oxytétracycline › Genomic insights into the antibiotic...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Mécanismes moléculaires de l'action pharmacologique › Antienzymes › Inhibiteurs de la synthèse protéique › Genomic insights into the antibiotic...