NGS in Hereditary Ataxia: When Rare Becomes Frequent.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
06 Aug 2021
Historique:
received: 23 06 2021
revised: 03 08 2021
accepted: 04 08 2021
entrez: 27 8 2021
pubmed: 28 8 2021
medline: 25 9 2021
Statut: epublish

Résumé

The term hereditary ataxia (HA) refers to a heterogeneous group of neurological disorders with multiple genetic etiologies and a wide spectrum of ataxia-dominated phenotypes. Massive gene analysis in next-generation sequencing has entered the HA scenario, broadening our genetic and clinical knowledge of these conditions. In this study, we employed a targeted resequencing panel (TRP) in a large and highly heterogeneous cohort of 377 patients with a clinical diagnosis of HA, but no molecular diagnosis on routine genetic tests. We obtained a positive result (genetic diagnosis) in 33.2% of the patients, a rate significantly higher than those reported in similar studies employing TRP (average 19.4%), and in line with those performed using exome sequencing (ES, average 34.6%). Moreover, 15.6% of the patients had an uncertain molecular diagnosis.

Identifiants

pubmed: 34445196
pii: ijms22168490
doi: 10.3390/ijms22168490
pmc: PMC8395181
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Ministero della Salute
ID : RF2016

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Auteurs

Daniele Galatolo (D)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Giovanna De Michele (G)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, 80131 Naples, Italy.

Gabriella Silvestri (G)

UOC Neurologia, Fondazione Policlinico Universitario 'A. Gemelli' IRCCS, 00168 Rome, Italy.
Department of Neurosciences, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.

Vincenzo Leuzzi (V)

Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy.

Carlo Casali (C)

Department of Medical and Surgical Sciences and Biotechnologies, Sapienza University of Rome, 40100 Latina, Italy.

Olimpia Musumeci (O)

Unit of Neurology and Neuromuscular Disorders, Department of Clinical and Experimental Medicine, University of Messina, 98122 Messina, Italy.

Antonella Antenora (A)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, 80131 Naples, Italy.

Guja Astrea (G)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Melissa Barghigiani (M)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Roberta Battini (R)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Carla Battisti (C)

Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy.

Caterina Caputi (C)

Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy.

Ettore Cioffi (E)

Department of Medical and Surgical Sciences and Biotechnologies, Sapienza University of Rome, 40100 Latina, Italy.

Giuseppe De Michele (G)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, 80131 Naples, Italy.

Maria Teresa Dotti (MT)

Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy.

Tommasina Fico (T)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, 80131 Naples, Italy.

Chiara Fiorillo (C)

Paediatric Neurology and Muscular Diseases Unit, University of Genoa and 'G. Gaslini' Institute, 16147 Genoa, Italy.

Serena Galosi (S)

Department of Human Neuroscience, Sapienza University of Rome, 00185 Rome, Italy.

Maria Lieto (M)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, 80131 Naples, Italy.

Alessandro Malandrini (A)

Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy.

Marina A B Melone (MAB)

Center for Rare Diseases and Interuniversity Center for Research in Neurosciences, Department of Advanced Medical and Surgical Sciences, 2nd Division of Neurology, University of Campania "Luigi Vanvitelli", 80131 Naples, Italy.

Andrea Mignarri (A)

Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 Siena, Italy.

Gemma Natale (G)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Elena Pegoraro (E)

Department of Neurosciences, University of Padua, 35121 Padua, Italy.

Antonio Petrucci (A)

Azienda Ospedaliera San Camillo-Forlanini, 00152 Rome, Italy.

Ivana Ricca (I)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Vittorio Riso (V)

UOC Neurologia, Fondazione Policlinico Universitario 'A. Gemelli' IRCCS, 00168 Rome, Italy.
Department of Neurosciences, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.

Salvatore Rossi (S)

UOC Neurologia, Fondazione Policlinico Universitario 'A. Gemelli' IRCCS, 00168 Rome, Italy.
Department of Neurosciences, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.

Anna Rubegni (A)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Arianna Scarlatti (A)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Francesca Tinelli (F)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Rosanna Trovato (R)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Gioacchino Tedeschi (G)

Center for Rare Diseases and Interuniversity Center for Research in Neurosciences, Department of Advanced Medical and Surgical Sciences, 2nd Division of Neurology, University of Campania "Luigi Vanvitelli", 80131 Naples, Italy.

Alessandra Tessa (A)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

Alessandro Filla (A)

Department of Neurosciences, Reproductive and Odontostomatological Sciences, Federico II University, 80131 Naples, Italy.

Filippo Maria Santorelli (FM)

Molecular Medicine for Neurodegenerative and Neuromuscular Diseases Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy.

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Classifications MeSH