Enrichment of provitamin A content in durum wheat grain by suppressing β-carotene hydroxylase 1 genes with a TILLING approach.
Carotenoids
Edible Grain
/ chemistry
Food, Fortified
Gene Knockout Techniques
Genotype
Metabolic Engineering
Mixed Function Oxygenases
/ genetics
Phylogeny
Plant Breeding
Provitamins
/ biosynthesis
Seeds
/ chemistry
Triticum
/ chemistry
Vitamin A
/ biosynthesis
Xanthophylls
Zeaxanthins
/ biosynthesis
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
ISSN: 1432-2242
Titre abrégé: Theor Appl Genet
Pays: Germany
ID NLM: 0145600
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
12
04
2021
accepted:
27
08
2021
pubmed:
4
9
2021
medline:
15
12
2021
entrez:
3
9
2021
Statut:
ppublish
Résumé
The suppression of the HYD-1 gene by a TILLING approach increases the amount of β-carotene in durum wheat kernel. Vitamin A deficiency is a major public health problem that affects numerous countries in the world. As humans are not able to synthesize vitamin A, it must be daily assimilated along with other micro- and macronutrients through the diet. Durum wheat is an important crop for Mediterranean countries and provides a discrete amount of nutrients, such as carbohydrates and proteins, but it is deficient in some essential micronutrients, including provitamin A. In the present work, a targeting induced local lesions in genomes strategy has been undertaken to obtain durum wheat genotypes biofortified in provitamin A. In detail, we focused on the suppression of the β-carotene hydroxylase 1 (HYD1) genes, encoding enzymes involved in the redirection of β-carotene toward the synthesis of the downstream xanthophylls (neoxanthin, violaxanthin and zeaxanthin). Expression analysis of genes involved in carotenoid biosynthesis revealed a reduction of the abundance of HYD1 transcripts greater than 50% in mutant grain compared to the control. The biochemical profiling of carotenoid in the wheat mutant genotypes highlighted a significant increase of more than 70% of β-carotene compared to the wild-type sibling lines, with no change in lutein, α-carotene and zeaxanthin content. This study sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat and provides new genotypes that represent a good genetic resource for future breeding programs focused on the provitamin A biofortification through non-transgenic approaches.
Identifiants
pubmed: 34477900
doi: 10.1007/s00122-021-03944-6
pii: 10.1007/s00122-021-03944-6
doi:
Substances chimiques
Provitamins
0
Xanthophylls
0
Zeaxanthins
0
Vitamin A
11103-57-4
Carotenoids
36-88-4
violaxanthin
51C926029A
Mixed Function Oxygenases
EC 1.-
beta-carotene hydroxylase
EC 1.14.13.-
neoxanthin
KK8M5T48AI
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4013-4024Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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