Properties of an acid-tolerant, persistent Cheddar cheese isolate, Lacticaseibacillus paracasei GCRL163.
Lactobacillus
Nutrient starvation
Proteomics
Tween 80
Journal
Journal of industrial microbiology & biotechnology
ISSN: 1476-5535
Titre abrégé: J Ind Microbiol Biotechnol
Pays: Germany
ID NLM: 9705544
Informations de publication
Date de publication:
23 Dec 2021
23 Dec 2021
Historique:
received:
13
08
2021
accepted:
11
09
2021
pubmed:
24
9
2021
medline:
25
12
2021
entrez:
23
9
2021
Statut:
ppublish
Résumé
The distinctive flavours in hard cheeses are attributed largely to the activity of nonstarter lactic acid bacteria (NSLAB) which dominate the cheese matrix during maturation after lactose is consumed. Understanding how different strains of NSLAB survive, compete, and scavenge available nutrients is fundamental to selecting strains as potential adjunct starters which may influence product traits. Three Lacticaseibacillus paracasei isolates which dominated at different stages over 63-week maturation periods of Australian Cheddar cheeses had the same molecular biotype. They shared many phenotypic traits, including salt tolerance, optimum growth temperature, growth on N-acetylglucosamine and N-acetylgalactosamine plus delayed growth on D-ribose, carbon sources likely present in cheese due to bacterial autolysis. However, strains 124 and 163 (later named GCRL163) survived longer at low pH and grew on D-tagatose and D-mannitol, differentiating this phenotype from strain 122. When cultured on growth-limiting lactose (0.2%, wt/vol) in the presence of high concentrations of L-leucine and other amino acids, GCRL163 produced, and subsequently consumed lactate, forming acetic and formic acids, and demonstrated temporal accumulation of intermediates in pyruvate metabolism in long-term cultures. Strain GCRL163 grew in Tween 80-tryptone broths, a trait not shared by all L. casei-group dairy isolates screened in this study. Including citrate in this medium stimulated growth of GCRL163 above citrate alone, suggesting cometabolism of citrate and Tween 80. Proteomic analysis of cytosolic proteins indicated that growth in Tween 80 produced a higher stress state and increased relative abundance of three cell envelope proteinases (CEPs) (including PrtP and Dumpy), amongst over 230 differentially expressed proteins.
Identifiants
pubmed: 34555172
pii: 6374559
doi: 10.1093/jimb/kuab070
pmc: PMC8788758
pii:
doi:
Substances chimiques
Lactic Acid
33X04XA5AT
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Dairy Australia
ID : ACTP029
Organisme : University of Tasmania
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology.
Références
J Dairy Sci. 2003 Sep;86(9):2773-82
pubmed: 14507013
Microorganisms. 2021 Jan 15;9(1):
pubmed: 33467737
J Proteome Res. 2013 Nov 1;12(11):5313-22
pubmed: 24066708
FEMS Microbiol Rev. 2017 Aug 1;41(Supp_1):S27-S48
pubmed: 28673043
Int J Food Microbiol. 2021 Jan 16;337:108937
pubmed: 33171308
Drugs Exp Clin Res. 2000;26(3):95-111
pubmed: 10941602
J Appl Microbiol. 2006 Oct;101(4):872-82
pubmed: 16968299
J Proteome Res. 2020 Apr 3;19(4):1824-1846
pubmed: 32108472
J Appl Microbiol. 2017 Mar;122(3):759-769
pubmed: 27981716
Genome Biol. 2007;8(2):R24
pubmed: 17324271
Compr Rev Food Sci Food Saf. 2021 Jan;20(1):369-400
pubmed: 33443792
Appl Microbiol Biotechnol. 2021 Jan;105(1):225-233
pubmed: 33215257
Appl Environ Microbiol. 1991 Dec;57(12):3535-40
pubmed: 16348602
Nucleic Acids Res. 2021 Jan 8;49(D1):D274-D281
pubmed: 33167031
Nucleic Acids Res. 2021 Jan 8;49(D1):D751-D763
pubmed: 33119741
FEMS Microbiol Lett. 2004 Oct 15;239(2):267-75
pubmed: 15476976
Microbiology (Reading). 2007 Aug;153(Pt 8):2655-2665
pubmed: 17660430
BMC Microbiol. 2018 Jul 13;18(1):72
pubmed: 30001697
Front Microbiol. 2018 Sep 10;9:2107
pubmed: 30298055
Syst Appl Microbiol. 2018 Jul;41(4):270-278
pubmed: 29456028
Int J Syst Evol Microbiol. 2020 Apr;70(4):2782-2858
pubmed: 32293557
Annu Rev Microbiol. 2009;63:269-90
pubmed: 19575569
J Appl Microbiol. 2001 Apr;90(4):600-8
pubmed: 11309072
PLoS One. 2013 Oct 08;8(10):e75073
pubmed: 24116025
Mol Microbiol. 2016 Apr;100(1):25-41
pubmed: 26711440
Sci Rep. 2017 Aug 17;7(1):8579
pubmed: 28819300
Int J Food Microbiol. 2012 Mar 15;154(3):162-8
pubmed: 22260926
Can J Microbiol. 1982 Mar;28(3):291-300
pubmed: 6805929
Front Mol Biosci. 2019 Aug 30;6:75
pubmed: 31544106
J Appl Microbiol. 2009 Mar;106(3):764-73
pubmed: 19302099
Appl Environ Microbiol. 2013 Aug;79(15):4603-12
pubmed: 23709502
Microbiol Mol Biol Rev. 2016 Jul 27;80(3):837-90
pubmed: 27466284
World J Microbiol Biotechnol. 2021 Mar 15;37(4):61
pubmed: 33719024
J Gen Microbiol. 1983 Oct;129(10):3227-37
pubmed: 6655460
Front Microbiol. 2018 Mar 23;9:535
pubmed: 29662477
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W592-5
pubmed: 15980543
Front Microbiol. 2018 Jul 05;9:1506
pubmed: 30026739
Microorganisms. 2019 Oct 25;7(11):
pubmed: 31731444
Anal Chem. 2003 Sep 1;75(17):4646-58
pubmed: 14632076
PLoS One. 2018 Oct 25;13(10):e0206317
pubmed: 30359441
J Proteome Res. 2018 Mar 2;17(3):1290-1299
pubmed: 29405720
J Appl Microbiol. 2017 May;122(5):1245-1261
pubmed: 28199757
Food Microbiol. 2012 Feb;29(1):1-9
pubmed: 22029912
Microbiology (Reading). 2007 Jan;153(Pt 1):291-9
pubmed: 17185558
Food Microbiol. 2020 Sep;90:103485
pubmed: 32336352
J Appl Microbiol. 2006 Jun;100(6):1171-85
pubmed: 16696665
Genome Announc. 2017 Nov 2;5(44):
pubmed: 29097471
J Dairy Sci. 2018 Apr;101(4):2887-2896
pubmed: 29428745