A Robust Platform for Unnatural Amino Acid Mutagenesis in E. coli Using the Bacterial Tryptophanyl-tRNA synthetase/tRNA pair.
BL21(DE3)
genetic code expansion
tryptophan
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
30 04 2022
30 04 2022
Historique:
received:
12
08
2021
revised:
04
10
2021
accepted:
06
10
2021
pubmed:
17
10
2021
medline:
19
4
2022
entrez:
16
10
2021
Statut:
ppublish
Résumé
We report the development of a robust user-friendly Escherichia coli (E. coli) expression system, derived from the BL21(DE3) strain, for site-specifically incorporating unnatural amino acids (UAAs) into proteins using engineered E. coli tryptophanyl-tRNA synthetase (EcTrpRS)-tRNA
Identifiants
pubmed: 34655653
pii: S0022-2836(21)00541-6
doi: 10.1016/j.jmb.2021.167304
pmc: PMC9005579
mid: NIHMS1754786
pii:
doi:
Substances chimiques
RNA, Transfer, Trp
0
Tryptophan
8DUH1N11BX
RNA, Transfer
9014-25-9
Tryptophan-tRNA Ligase
EC 6.1.1.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
167304Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM136437
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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