The proteome of developing barley anthers during meiotic prophase I.
Hordeum vulgare
Anthers
barley
meiotic prophase I
proteome
proteomics
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
02 03 2022
02 03 2022
Historique:
received:
02
09
2021
accepted:
08
11
2021
pubmed:
11
11
2021
medline:
11
3
2022
entrez:
10
11
2021
Statut:
ppublish
Résumé
Flowering plants reproduce sexually by combining a haploid male and female gametophyte during fertilization. Male gametophytes are localized in the anthers, each containing reproductive (meiocyte) and non-reproductive tissue necessary for anther development and maturation. Meiosis, where chromosomes pair and exchange their genetic material during a process called recombination, is one of the most important and sensitive stages in breeding, ensuring genetic diversity. Most anther development studies have focused on transcript variation, but very few have been correlated with protein abundance. Taking advantage of a recently published barley anther transcriptomic (BAnTr) dataset and a newly developed sensitive mass spectrometry-based approach to analyse the barley anther proteome, we conducted high-resolution mass spectrometry analysis of barley anthers, collected at six time points and representing their development from pre-meiosis to metaphase. Each time point was carefully staged using immunocytology, providing a robust and accurate staging mirroring our previous BAnTr dataset. We identified >6100 non-redundant proteins including 82 known and putative meiotic proteins. Although the protein abundance was relatively stable throughout prophase I, we were able to quantify the dynamic variation of 336 proteins. We present the first quantitative comparative proteomics study of barley anther development during meiotic prophase I when the important process of homologous recombination is taking place.
Identifiants
pubmed: 34758083
pii: 6425115
doi: 10.1093/jxb/erab494
pmc: PMC8890616
doi:
Substances chimiques
Plant Proteins
0
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1464-1482Subventions
Organisme : European Research Council
ID : 669182
Pays : International
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/T008636/1
Pays : United Kingdom
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Références
Plant Cell. 2007 Nov;19(11):3549-62
pubmed: 18032630
J Integr Plant Biol. 2015 Nov;57(11):876-91
pubmed: 26310290
Mol Genet Genomics. 2004 Mar;271(2):121-9
pubmed: 14758540
Genetics. 2016 Oct;204(2):543-553
pubmed: 27466226
Nat Rev Mol Cell Biol. 2015 Dec;16(12):727-41
pubmed: 26530390
Plant J. 2015 Nov;84(3):527-44
pubmed: 26360816
Plant J. 2014 May;78(3):385-97
pubmed: 24635777
Plant Cell. 2003 Aug;15(8):1728-39
pubmed: 12897248
Plant J. 2008 Jul;55(2):266-77
pubmed: 18397379
Front Plant Sci. 2016 Jun 02;7:762
pubmed: 27313591
Plant Cell. 2006 Jul;18(7):1667-80
pubmed: 16751349
Plant Physiol. 2015 Apr;167(4):1717-30
pubmed: 25667314
Front Plant Sci. 2014 Jul 21;5:347
pubmed: 25101101
Plant Physiol. 2017 Jul;174(3):1779-1794
pubmed: 28515146
BMC Plant Biol. 2014 May 03;14:118
pubmed: 24885405
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3146-51
pubmed: 25713378
Front Plant Sci. 2018 May 11;9:584
pubmed: 29868052
Plant Cell. 2006 Nov;18(11):2999-3014
pubmed: 17138695
J Exp Bot. 2016 Sep;67(17):4993-5008
pubmed: 27531888
Plant Cell. 2011 Dec;23(12):4266-79
pubmed: 22202891
Plant Cell. 2019 Feb;31(2):444-464
pubmed: 30674694
Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20240-5
pubmed: 19910535
Plant J. 2002 Aug;31(4):517-28
pubmed: 12182708
BMC Plant Biol. 2010 Dec 17;10:280
pubmed: 21167045
Bioinformatics. 2006 Jul 1;22(13):1600-7
pubmed: 16606683
J Exp Bot. 2021 Apr 2;72(8):3012-3027
pubmed: 33502451
Curr Biol. 2005 Nov 8;15(21):1919-25
pubmed: 16271868
EMBO J. 2009 Jan 21;28(2):99-111
pubmed: 19078966
Proteomics. 2003 May;3(5):738-51
pubmed: 12748952
Biol Reprod. 2012 Sep 13;87(3):62
pubmed: 22674395
Cell. 2009 May 1;137(3):498-508
pubmed: 19410546
Plant J. 2018 Jan;93(1):142-154
pubmed: 29124795
Plant Cell. 2014 Apr 22;26(4):1512-1524
pubmed: 24755456
FEBS J. 2015 Jul;282(13):2471-87
pubmed: 25470754
Plant J. 2011 Feb;65(4):503-16
pubmed: 21208307
Plant Cell Rep. 2020 Sep;39(9):1185-1197
pubmed: 32638075
Nat Commun. 2021 Jul 9;12(1):4212
pubmed: 34244496
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Plant J. 2003 Jan;33(2):413-23
pubmed: 12535353
Plant Cell. 2012 Oct;24(10):4096-109
pubmed: 23104831
Plant Physiol. 2018 Feb;176(2):1610-1626
pubmed: 29187566
J Cell Sci. 2002 Sep 15;115(Pt 18):3645-55
pubmed: 12186950
J Proteome Res. 2011 Apr 1;10(4):1794-805
pubmed: 21254760
J Cell Biol. 2018 Dec 3;217(12):4070-4079
pubmed: 30266762
Genes Dev. 2002 Aug 1;16(15):2021-31
pubmed: 12154130
J Exp Bot. 2011 Mar;62(5):1565-79
pubmed: 21081662
Methods Mol Biol. 2020;2061:367-379
pubmed: 31583673
Annu Rev Plant Biol. 2005;56:393-434
pubmed: 15862102
Plant Cell. 2021 Aug 31;33(8):2685-2700
pubmed: 34003932
Genes Dev. 2015 Oct 15;29(20):2183-202
pubmed: 26494791
Development. 2006 Aug;133(16):3085-95
pubmed: 16831835
Genes Dev. 2007 Sep 1;21(17):2220-33
pubmed: 17785529
Front Plant Sci. 2014 Jul 24;5:356
pubmed: 25104955
Plant Cell. 1993 Oct;5(10):1217-29
pubmed: 8281038
Front Plant Sci. 2019 Apr 02;10:393
pubmed: 31001307
Plant J. 2012 Apr;70(2):256-70
pubmed: 22111585
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14487-92
pubmed: 14612572
PLoS One. 2010 Dec 31;5(12):e15770
pubmed: 21209834
Mol Cell Proteomics. 2014 Jan;13(1):295-310
pubmed: 24078888
Plant Signal Behav. 2010 Feb;5(2):168-70
pubmed: 20023430
Plant Physiol. 2012 Oct;160(2):990-9
pubmed: 22846193
J Cell Biol. 2012 Jan 23;196(2):233-46
pubmed: 22249291
Plant Physiol. 2020 Nov;184(3):1407-1423
pubmed: 32917771
Proc Natl Acad Sci U S A. 2012 May 29;109(22):8374-81
pubmed: 22592791
Nucleic Acids Res. 2021 Jan 25;49(2):879-890
pubmed: 33406239
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Int J Mol Sci. 2019 Aug 15;20(16):
pubmed: 31443183
NPJ Syst Biol Appl. 2019 Apr 4;5:12
pubmed: 30962948
Front Plant Sci. 2021 Jan 12;11:619404
pubmed: 33510760
J Proteomics. 2021 Feb 10;232:104026
pubmed: 33127528
Nat Genet. 2018 Jan;50(1):130-137
pubmed: 29255257
Plant Cell. 2011 Feb;23(2):515-33
pubmed: 21297036
Plant J. 2012 Sep;71(5):800-9
pubmed: 22519817
Plant Physiol. 2017 Jun;174(2):1205-1215
pubmed: 28455402
Front Plant Sci. 2017 Jul 13;8:1235
pubmed: 28751906
Front Plant Sci. 2014 Jun 16;5:277
pubmed: 24982667
Nat Commun. 2020 Nov 27;11(1):6031
pubmed: 33247135
Nucleic Acids Res. 2017 Aug 21;45(14):8551-8563
pubmed: 28575480
Plant Physiol. 2010 Nov;154(3):1492-504
pubmed: 20805327
Mol Cells. 2013 Mar;35(3):202-9
pubmed: 23456296
Cell. 2014 Apr 24;157(3):624-35
pubmed: 24766808
Proc Natl Acad Sci U S A. 2019 Apr 9;116(15):7549-7558
pubmed: 30902896
Cell Cycle. 2008 Mar 15;7(6):741-4
pubmed: 18239464
J Exp Bot. 2016 Nov;67(21):6037-6049
pubmed: 27702997
Plant Cell. 2007 Aug;19(8):2583-94
pubmed: 17675402
Plant Cell. 2010 Oct;22(10):3232-48
pubmed: 21037105
Mol Cell. 2013 Jan 10;49(1):43-54
pubmed: 23246437
Int J Genomics. 2015;2015:238704
pubmed: 26618163
Nucleic Acids Res. 2018 Jul 2;46(W1):W84-W88
pubmed: 29741643
Plant J. 2007 Nov;52(3):528-38
pubmed: 17727613
Trends Genet. 2018 Mar;34(3):232-245
pubmed: 29290403
Plant J. 2017 Dec;92(6):1076-1091
pubmed: 29031031