Direct RNA Nanopore Sequencing of Pseudomonas aeruginosa Clone C Transcriptomes.
Nanopore sequencing
Pseudomonas aeruginosa
antisense RNA
direct RNA sequencing
tRNA
transcriptome
Journal
Journal of bacteriology
ISSN: 1098-5530
Titre abrégé: J Bacteriol
Pays: United States
ID NLM: 2985120R
Informations de publication
Date de publication:
18 01 2022
18 01 2022
Historique:
pubmed:
16
11
2021
medline:
11
2
2022
entrez:
15
11
2021
Statut:
ppublish
Résumé
The transcriptomes of Pseudomonas aeruginosa clone C isolates NN2 and SG17M during the mid-exponential and early stationary phases of planktonic growth were evaluated by direct RNA sequencing on the nanopore platform and compared with established short-read cDNA sequencing on the Illumina platform. Fifty to ninety percent of the sense RNAs turned out to be rRNA molecules, followed by similar proportions of mRNA transcripts and noncoding RNAs. The two platforms detected similar proportions of uncharged tRNAs and 29 yet-undescribed antisense tRNAs. For example, the rarest arginine codon was paired with the most abundant tRNA
Identifiants
pubmed: 34780302
doi: 10.1128/JB.00418-21
pmc: PMC8765398
doi:
Substances chimiques
RNA, Bacterial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0041821Références
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