Evaluating Large Spontaneous Deletions in a Bovine Cell Line Selected for Bovine Viral Diarrhea Virus Resistance.
Animals
CRISPR-Cas Systems
Cell Line
Diarrhea
/ virology
Diarrhea Viruses, Bovine Viral
/ physiology
Dogs
GTPase-Activating Proteins
/ genetics
Gene Deletion
Gene Knockout Techniques
Nerve Tissue Proteins
/ genetics
Protein Tyrosine Phosphatase, Non-Receptor Type 12
/ genetics
Receptors, Glutamate
/ genetics
Virus Internalization
Virus Replication
Whole Genome Sequencing
BVDV
CRIB
CRISPR
GRID2
MDBK
PTPN12
RABGAP1L
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
25 10 2021
25 10 2021
Historique:
received:
22
09
2021
revised:
20
10
2021
accepted:
22
10
2021
entrez:
27
11
2021
pubmed:
28
11
2021
medline:
15
2
2022
Statut:
epublish
Résumé
Bovine viral diarrhea virus's (BVDV) entry into bovine cells involves attachment of virions to cellular receptors, internalization, and pH-dependent fusion with endosomal membranes. The primary host receptor for BVDV is CD46; however, the complete set of host factors required for virus entry is unknown. The Madin-Darby bovine kidney (MDBK) cell line is susceptible to BVDV infection, while a derivative cell line (CRIB) is resistant at the level of virus entry. We performed complete genome sequencing of each to identify genomic variation underlying the resistant phenotype with the aim of identifying host factors essential for BVDV entry. Three large compound deletions in the BVDV-resistant CRIB cell line were identified and predicted to disrupt the function or expression of the genes
Identifiants
pubmed: 34834954
pii: v13112147
doi: 10.3390/v13112147
pmc: PMC8622392
pii:
doi:
Substances chimiques
GTPase-Activating Proteins
0
Nerve Tissue Proteins
0
Receptors, Glutamate
0
Protein Tyrosine Phosphatase, Non-Receptor Type 12
EC 3.1.3.48
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Can J Comp Med. 1984 Apr;48(2):156-61
pubmed: 6326980
J Vet Intern Med. 2020 Sep;34(5):1690-1706
pubmed: 32633084
J Virol. 2004 Feb;78(4):1792-9
pubmed: 14747544
J Gen Virol. 1996 Jun;77 ( Pt 6):1295-303
pubmed: 8683219
Autophagy. 2014 Jul;10(7):1154-66
pubmed: 24915298
J Virol. 2017 Jan 3;91(2):
pubmed: 27847356
Gigascience. 2020 Mar 1;9(3):
pubmed: 32191811
Pharmacol Res. 2019 Jun;144:73-78
pubmed: 30959160
BMC Bioinformatics. 2017 Jan 31;18(1):73
pubmed: 28143617
J Vet Diagn Invest. 2016 Sep;28(5):519-28
pubmed: 27400958
Elife. 2016 Oct 08;5:
pubmed: 27718357
J Virol. 2000 Oct;74(20):9553-61
pubmed: 11000226
J Neurosci. 2011 Mar 2;31(9):3362-74
pubmed: 21368048
Nat Biotechnol. 2013 Mar;31(3):213-9
pubmed: 23396013
Transbound Emerg Dis. 2019 Mar;66(2):640-652
pubmed: 30415496
Genome Res. 2010 Sep;20(9):1297-303
pubmed: 20644199
Vet J. 2017 Feb;220:80-87
pubmed: 28190502
Nat Biotechnol. 2016 May 6;34(5):479-81
pubmed: 27153274
J Gen Virol. 2017 Jan;98(1):2-3
pubmed: 28218572
J Gen Virol. 2000 Feb;81(Pt 2):451-9
pubmed: 10644844
Nat Rev Immunol. 2019 Jul;19(7):417-432
pubmed: 30918351
Bioinformatics. 2015 Dec 15;31(24):4014-6
pubmed: 26358729
Virology. 1995 Apr 20;208(2):565-75
pubmed: 7747428
J Virol. 2006 Apr;80(8):3912-22
pubmed: 16571808
PLoS Pathog. 2017 Feb 23;13(2):e1006206
pubmed: 28231264
Biologicals. 2013 Jan;41(1):14-9
pubmed: 22883306
J Virol. 2005 Apr;79(7):4191-200
pubmed: 15767420
PLoS Genet. 2019 Jan 24;15(1):e1007902
pubmed: 30677042
Am J Vet Res. 2009 Jan;70(1):73-85
pubmed: 19119951
Genome Biol. 2010;11(3):R25
pubmed: 20196867
J Virol. 2006 Nov;80(21):10862-7
pubmed: 16928760
J Vet Diagn Invest. 2002 Mar;14(2):120-5
pubmed: 11939332
J Gen Virol. 2002 Sep;83(Pt 9):2153-2159
pubmed: 12185268
Viruses. 2020 Aug 06;12(8):
pubmed: 32781607
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
F1000Res. 2016 Aug 16;5:2003
pubmed: 27746904
Cornell Vet. 1946 Jul;36:205-13
pubmed: 20995890
Reprod Fertil Dev. 2013;26(1):74-82
pubmed: 24305179
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Cell. 2014 Jun 5;157(6):1262-1278
pubmed: 24906146
Science. 2014 Nov 28;346(6213):1258096
pubmed: 25430774
J Virol. 2005 Aug;79(16):10826-9
pubmed: 16051874
Genome Res. 2012 Mar;22(3):568-76
pubmed: 22300766
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943