Expression of microbial rhodopsins in
Biotechnology and bioengineering
Cell Membrane
Protein Biochemistry
Protein expression and purification
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 03 2022
18 03 2022
Historique:
entrez:
5
1
2022
pubmed:
6
1
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Microbial rhodopsins are photoreceptive membrane proteins showing various light-dependent biological activities. Styrene-maleic acid (SMA) copolymers spontaneously form nanoscale lipid particles containing membrane proteins and associated lipids without detergent, and can be used to characterize membrane molecules. Here, we provide a protocol to functionally express a thermally stable rhodopsin,
Identifiants
pubmed: 34984357
doi: 10.1016/j.xpro.2021.101046
pii: S2666-1667(21)00752-8
pmc: PMC8693275
doi:
Substances chimiques
Maleates
0
Membrane Proteins
0
Rhodopsins, Microbial
0
Rhodopsin
9009-81-8
maleic acid
91XW058U2C
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101046Informations de copyright
© 2021.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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