Bio-Molecular analysis of selected food derived Lactiplantibacillus strains for CLA production reveals possibly a complex mechanism.


Journal

Food research international (Ottawa, Ont.)
ISSN: 1873-7145
Titre abrégé: Food Res Int
Pays: Canada
ID NLM: 9210143

Informations de publication

Date de publication:
04 2022
Historique:
received: 15 12 2021
revised: 14 02 2022
accepted: 15 02 2022
entrez: 26 3 2022
pubmed: 27 3 2022
medline: 8 4 2022
Statut: ppublish

Résumé

Lactiplantibacillus is among the most extensively studied bacterial specie belonging to the genus Lactobacillus with proven probiotic and health promoting effects. These beneficial effects are generally strains specific but the underlying molecular mechanisms are still not fully understood. Dissecting the determinants behind probiotic topographies of this bacterium is of particular interest since it would help select strains that stand the best chance of success in clinical trials and potential industrial applications. In the current study, we have compared the oleate hydratase phylogeny of seven selected strains of L. plantarum on the basis of their sequence, physiochemical properties and 3D structures. All the strains were assessed on molecular level visualization to find out the active site residues which take part in binding with linoleic acid (LA) at the time of interactions. From the whole genome sequences, the genes responsible for conjugated linoleic acid (CLA) production were identified by Venn diagrams. Identified genes were further compared phylogenetically by MEGA X and physiochemical parameters were analyzed by utilizing ExPaSy-Protparam. The genes were further analyzed for the secondary structures using PSIPRED and tertiary structure was generated by trRosseta and SWISS-MODEL. For the analysis of molecular interactions, LA was used as a ligand and the docking was performed using AutoDock Vina. The phylogenetic analysis showed a close phylogeny of the strains with publicly available genomes. The best interaction energy with LA was observed as -6.7 kcal/mol. The bacteria perform an important role in the CLA production through LA metabolism. Oleate hydratase genes are involved in the complex mechanism of the saturated conversion of LA in to CLA. The current study provides further insights for CLA production by different strains of L. plantarum. There is an excellent opportunity for future studies to investigate different CLA production mechanisms in different bacterial strains.

Identifiants

pubmed: 35337595
pii: S0963-9969(22)00088-6
doi: 10.1016/j.foodres.2022.111031
pii:
doi:

Substances chimiques

Linoleic Acids, Conjugated 0
Linoleic Acid 9KJL21T0QJ

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

111031

Informations de copyright

Copyright © 2022 Elsevier Ltd. All rights reserved.

Auteurs

Tariq Aziz (T)

School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Basic Medical Sciences, Khyber Medical University, Peshawar 25120, Pakistan.

Abid Sarwar (A)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China.

Muhammad Naveed (M)

Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore 54590, Pakistan.

Muhammad Shahzad (M)

Institute of Basic Medical Sciences, Khyber Medical University, Peshawar 25120, Pakistan.

Muhammad Aqib Shabbir (M)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China.

Anas S Dablool (AS)

Department of Public Health, Health Sciences College Al-Leith, Umm Al-Qura University, Makkah al-Mukarramah 24382, Saudi Arabia.

Jalal Ud Din (J)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China.

Ayaz Ali Khan (A)

Department of Biotechnology, University of Malakand, Chakdara 18800, Pakistan.

Sumaira Naz (S)

Department of Biochemistry, University of Malakand, Chakdara 18800, Pakistan.

Haiying Cui (H)

School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: cuihaiying@ujs.edu.cn.

Lin Lin (L)

School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: linl@ujs.edu.cn.

Articles similaires

Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing
Animals Hemiptera Insect Proteins Phylogeny Insecticides
Amaryllidaceae Alkaloids Lycoris NADPH-Ferrihemoprotein Reductase Gene Expression Regulation, Plant Plant Proteins
Drought Resistance Gene Expression Profiling Gene Expression Regulation, Plant Gossypium Multigene Family

Classifications MeSH