Advances in the application of fluorescence correlation spectroscopy to study detergent purified and encapsulated membrane proteins.

Detergents Fluorescence Lipid Bilayers / chemistry Membrane Proteins / chemistry Polystyrenes / chemistry
Detergent extraction Fluorescence correlation spectroscopy Ligand binding Membrane protein SMALP

Journal

The international journal of biochemistry & cell biology
ISSN: 1878-5875
Titre abrégé: Int J Biochem Cell Biol
Pays: Netherlands
ID NLM: 9508482

Informations de publication

Date de publication:
05 2022
Historique:
received: 13 12 2021
revised: 30 03 2022
accepted: 01 04 2022
pubmed: 8 4 2022
medline: 3 5 2022
entrez: 7 4 2022
Statut: ppublish

Résumé

Fluorescence correlation spectroscopy (FCS) is a quantitative spectroscopy technique which could potentially increase throughput and sensitivity of screening for ligand, substrate and inhibitor binding to membrane proteins in solution. However, the purification of membrane proteins in their active forms is complex, as the lipid bilayer provides stability and its removal often causes the protein to become conformationally unstable. This has limited the application of biophysical techniques such as FCS to study the function of membrane proteins. The recent application of native extraction techniques such as styrene maleic acid lipid particles (SMALPs) has resolved this issue and FCS has emerged as a powerful option for studying proteins extracted in this way. This review will discuss the application of FCS to study purified membrane proteins in detergent micelles, nanodiscs and SMALPs and its potential to be used routinely in membrane protein drug discovery.

Identifiants

DOI: 10.1016/j.biocel.2022.106210 PMID: 35390493
pubmed: 35390493
pii: S1357-2725(22)00055-3
doi: 10.1016/j.biocel.2022.106210
pii:
doi:

Substances chimiques

Detergents 0
Lipid Bilayers 0
Membrane Proteins 0
Polystyrenes 0

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

106210

Subventions

Organisme : Medical Research Council
ID : MR/N020081/1
Pays : United Kingdom

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.

Auteurs

Leigh A Stoddart (LA)

Cell Signalling and Pharmacology Research Group, Division of Physiology Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, NG7 2UH, UK; Centre of Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, The Midlands, UK.

Joëlle Goulding (J)

Cell Signalling and Pharmacology Research Group, Division of Physiology Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, NG7 2UH, UK; Centre of Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, The Midlands, UK.

Stephen J Briddon (SJ)

Cell Signalling and Pharmacology Research Group, Division of Physiology Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, NG7 2UH, UK; Centre of Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, The Midlands, UK. Electronic address: Stephen.briddon@nottingham.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits domestiques › Détergents › Advances in the application of fluorescence...
questionsmedicales.fr › Phénomènes physiques › Phénomènes optiques › Fluorescence › Advances in the application of fluorescence...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Double couche lipidique › Advances in the application of fluorescence...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Advances in the application of fluorescence...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Polystyrènes › Advances in the application of fluorescence...