Production Optimization and Biochemical Characterization of Cellulase from
Journal
BioMed research international
ISSN: 2314-6141
Titre abrégé: Biomed Res Int
Pays: United States
ID NLM: 101600173
Informations de publication
Date de publication:
2022
2022
Historique:
received:
24
12
2021
revised:
11
04
2022
accepted:
16
04
2022
entrez:
23
5
2022
pubmed:
24
5
2022
medline:
25
5
2022
Statut:
epublish
Résumé
This study is aimed at isolating and identifying a thermophilic cellulolytic bacterium from hot spring water and characterizing thermostable cellulase produced by the isolate. Enrichment and culture of water sample was used for isolation of bacterial strains and an isolate with highest cellulase activity was chosen for the production, partial purification, and biochemical characterization of the enzyme. Different staining techniques, enzymatic tests, and 16s ribosomal DNA (16s rDNA) gene sequencing were used for the identification of the isolate. The cellulase producing isolate was Gram positive, motile, and sporulated rod-shaped bacterium growing optimally between 55°C and 65°C. Based on partial 16s rDNA sequence analysis, the isolate was identified as
Identifiants
pubmed: 35601142
doi: 10.1155/2022/6840409
pmc: PMC9119783
doi:
Substances chimiques
DNA, Ribosomal
0
Water
059QF0KO0R
Cellulase
EC 3.2.1.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6840409Informations de copyright
Copyright © 2022 Subash Khadka et al.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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