Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq.
Acute lymphoblastic leukemia
Gene rearrangements
Immunoglobulin
Marker identification
Minimal residual disease
RNA-Seq
T-cell receptor
Whole exome sequencing
Whole genome sequencing
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
27
5
2022
pubmed:
28
5
2022
medline:
1
6
2022
Statut:
ppublish
Résumé
Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma.
Identifiants
pubmed: 35622320
doi: 10.1007/978-1-0716-2115-8_4
pmc: PMC9761498
doi:
Substances chimiques
Immunoglobulins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-77Informations de copyright
© 2022. The Author(s).
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