Characterization of 58 STRs and 94 SNPs with the ForenSeq™ DNA signature prep kit in Mexican-Mestizos from the Monterrey city (Northeast, Mexico).
ForenSeq DNA signature Prep
MPS
Mexican population
MiSeq FGx
Verogen
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
received:
26
10
2021
accepted:
06
05
2022
pubmed:
4
6
2022
medline:
26
7
2022
entrez:
3
6
2022
Statut:
ppublish
Résumé
STR allele frequency databases from populations are necessary to take full advantage of the increased power of discrimination offered by massively parallel sequencing (MPS) platforms. For this reason, we sequenced 58 STRs (aSTRs, X-STRs, and Y-STRs) and 94 identity informative SNPs (iiSNPs) on 105 Mestizo (admixed) individuals from Monterrey City (Northeast, Mexico), with the Primer Set-A of the ForenSeq™ DNA Signature Prep Kit. Most of the STR markers were in Hardy Weinberg equilibrium, with a few exceptions. We found 346 different length-based alleles for these 58 STRs; nevertheless, they became 528 alleles when the sequence was assessed. The combined power of discrimination from autosomal STRs (aSTRs) was -virtually- 100% in both length and sequence-based alleles, while the power of exclusion was 99.9999999976065 and 99.9999999999494%, respectively. Haplotypes based on X-STRs and Y-STRs showed 100% of discriminatory capacity. These results provide -for the first time- forensic genomic population data from Mexico necessary for interpretation in kinship and criminal analyses.
Sections du résumé
BACKGROUND
BACKGROUND
STR allele frequency databases from populations are necessary to take full advantage of the increased power of discrimination offered by massively parallel sequencing (MPS) platforms.
MATERIAL AND METHODS
METHODS
For this reason, we sequenced 58 STRs (aSTRs, X-STRs, and Y-STRs) and 94 identity informative SNPs (iiSNPs) on 105 Mestizo (admixed) individuals from Monterrey City (Northeast, Mexico), with the Primer Set-A of the ForenSeq™ DNA Signature Prep Kit.
RESULTS
RESULTS
Most of the STR markers were in Hardy Weinberg equilibrium, with a few exceptions. We found 346 different length-based alleles for these 58 STRs; nevertheless, they became 528 alleles when the sequence was assessed. The combined power of discrimination from autosomal STRs (aSTRs) was -virtually- 100% in both length and sequence-based alleles, while the power of exclusion was 99.9999999976065 and 99.9999999999494%, respectively. Haplotypes based on X-STRs and Y-STRs showed 100% of discriminatory capacity.
CONCLUSIONS
CONCLUSIONS
These results provide -for the first time- forensic genomic population data from Mexico necessary for interpretation in kinship and criminal analyses.
Identifiants
pubmed: 35657453
doi: 10.1007/s11033-022-07575-y
pii: 10.1007/s11033-022-07575-y
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7601-7609Subventions
Organisme : Consejo Nacional de Ciencia y Tecnología
ID : 286623
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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