Phage long tail fiber protein-immobilized magnetic nanoparticles for rapid and ultrasensitive detection of Salmonella.

Bacteriophages Food Microbiology Immunomagnetic Separation / methods Magnetite Nanoparticles Polymerase Chain Reaction Salmonella typhimurium / genetics
Bio-separation Direct PCR Magnetic nanocomposite Phage tail fiber proteins (TFPs) Salmonella detection

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Oct 2022
Historique:
received: 16 02 2022
revised: 26 05 2022
accepted: 28 05 2022
pubmed: 7 6 2022
medline: 22 6 2022
entrez: 6 6 2022
Statut: ppublish

Résumé

There is an urgent need to develop fast and sensitive detection methods for foodborne pathogens. But the conventional culture method that typically requires 2-3 days is not ideal for the rapid analysis. Food samples demonstrate a great challenge for direct detection due to the complex matrix. Hence, we present a new method based on the phage long-tail-fiber proteins (LTF4-a) immobilized magnetic nanoparticles (MNPs) for specific separation and concentration of Salmonella. The LTF4-a-MNP was prepared via the coupling of recombinant LTF4-a with MNPs and used to isolate and enrich Salmonella cells from contaminated food samples. The captured material was further integrated with the direct PCR program for accurate detection of Salmonella. Our study successfully established a new method for detecting contaminated food samples of Salmonella, the overall approach took no more than 3 h, which allowed a detection limit of 7 CFU/mL, demonstrating a promising alternative to the immunomagnetic separation method by replacing antibodies or aptamers, that is compatible with downstream analysis.

Identifiants

DOI: 10.1016/j.talanta.2022.123627 PMID: 35661002
pubmed: 35661002
pii: S0039-9140(22)00423-4
doi: 10.1016/j.talanta.2022.123627
pii:
doi:

Substances chimiques

Magnetite Nanoparticles 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

123627

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Luokai Wang (L)

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province, 266003, PR China.

Hong Lin (H)

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province, 266003, PR China.

Jing Zhang (J)

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province, 266003, PR China.

Jingxue Wang (J)

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong Province, 266003, PR China. Electronic address: snow@ouc.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Virus › Bactériophages › Phage long tail fiber protein-immobilized...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Qualité alimentaire › Sécurité des aliments › Contamination des aliments › Microbiologie alimentaire › Phage long tail fiber protein-immobilized...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Séparation cellulaire › Séparation immunomagnétique › Phage long tail fiber protein-immobilized...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Nanoparticules métalliques › Nanoparticules magnétiques d'oxyde de fer › Nanoparticules de magnétite › Phage long tail fiber protein-immobilized...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Phage long tail fiber protein-immobilized...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Salmonella › Salmonella enterica › Salmonella typhimurium › Phage long tail fiber protein-immobilized...