Pseudomonas aeruginosa Ld-08 isolated from Lilium davidii exhibits antifungal and growth-promoting properties.

Antifungal Agents Lilium / genetics Phylogeny Plant Diseases / microbiology Pseudomonas aeruginosa / genetics RNA, Ribosomal, 16S / genetics

Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2022
Historique:
received: 01 01 2022
accepted: 24 05 2022
entrez: 17 6 2022
pubmed: 18 6 2022
medline: 22 6 2022
Statut: epublish

Résumé

A plant growth-promoting and antifungal endophytic bacteria designated as Ld-08 isolated from the bulbs of Lilium davidii was identified as Pseudomonas aeruginosa based on phenotypic, microscopic, and 16S rRNA gene sequence analysis. Ld-08 exhibited antifungal effects against Fusarium oxysporum, Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi. Ld-08 showed the highest growth inhibition, i.e., 83.82±4.76% against B. dothidea followed by 74.12±3.87%, 67.56±3.35%, and 63.67±3.39% against F. fujikuroi, B. cinerea, and F. oxysporum, respectively. The ethyl acetate fraction of Ld-08 revealed the presence of several bioactive secondary metabolites. Prominent compounds were quinolones; 3,9-dimethoxypterocarpan; cascaroside B; dehydroabietylamine; epiandrosterone; nocodazole; oxolinic acid; pyochelin; rhodotulic acid; 9,12-octadecadienoic acid; di-peptides; tri-peptides; ursodiol, and venlafaxine. The strain Ld-08 showed organic acids, ACC deaminase, phosphate solubilization, IAA, and siderophore. The sterilized bulbs of a Lilium variety, inoculated with Ld-08, were further studied for plant growth-promoting traits. The inoculated plants showed improved growth than the control plants. Importantly, some growth parameters such as plant height, leaf length, bulb weight, and root length were significantly (P ≤0.05) increased in the inoculated plants than in the control un-inoculated plants. Further investigations are required to explore the potential of this strain to be used as a plant growth-promoting and biocontrol agent in sustainable agriculture.

Identifiants

DOI: 10.1371/journal.pone.0269640 PMID: 35714148 PMC: PMC9205524
pubmed: 35714148
doi: 10.1371/journal.pone.0269640
pii: PONE-D-22-00033
pmc: PMC9205524
doi:

Substances chimiques

Antifungal Agents 0
RNA, Ribosomal, 16S 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0269640

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Mohammad Sayyar Khan (MS)

Microbiology Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar, Khyber Pakhtunkhwa, Pakistan.
Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
ORCID: 0000-0001-9319-6222

Junlian Gao (J)

Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Mingfang Zhang (M)

Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Jing Xue (J)

Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Xiuhai Zhang (X)

Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antifongiques › Pseudomonas aeruginosa Ld-08 isolated from...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Lilianae › Liliales › Liliaceae › Lilium › Pseudomonas aeruginosa Ld-08 isolated from...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Pseudomonas aeruginosa Ld-08 isolated from...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Maladies des plantes › Pseudomonas aeruginosa Ld-08 isolated from...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa › Pseudomonas aeruginosa Ld-08 isolated from...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Pseudomonas aeruginosa Ld-08 isolated from...