An Assay for the Seeding of Homotypic Pyrin Domain Filament Transitions.

Caspase 1 / metabolism Cytoskeletal Proteins / metabolism NLR Family, Pyrin Domain-Containing 3 Protein / metabolism Protein Binding Pyrin Domain
ASC Electron microscopy Filament elongation Filament nucleation Inflammasome Innate immunity NLRP3 PYD Pyrin domain

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 27 6 2022
pubmed: 28 6 2022
medline: 30 6 2022
Statut: ppublish

Résumé

Pattern recognition receptors of innate immune cells allow the recognition of invariant microbial structures. The nucleotide-binding oligomerization domain-like receptors (NLRs) comprise 22 members, divided into 3 subfamilies. Homotypic pyrin domain (PYD) interactions were shown to mediate the interaction of inflammasome forming NLRPs with the adaptor protein ASC, bridging the interaction to caspase-1 and resulting in caspase-1-induced cytokine maturation and pyroptotic cell death. Here we describe a NLRP3

Identifiants

DOI: 10.1007/978-1-0716-2449-4_13 PMID: 35759199
pubmed: 35759199
doi: 10.1007/978-1-0716-2449-4_13
doi:

Substances chimiques

Cytoskeletal Proteins 0
NLR Family, Pyrin Domain-Containing 3 Protein 0
Caspase 1 EC 3.4.22.36

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

197-207

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Martinon F, Burns K, Tschopp J (2002) The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell 10:417–426. https://doi.org/10.1016/s1097-2765(02)00599-3
doi: 10.1016/s1097-2765(02)00599-3 pubmed: 12191486
Kufer TA, Sansonetti PJ (2011) NLR functions beyond pathogen recognition. Nat Immunol 12:121–128. https://doi.org/10.1038/ni.1985
doi: 10.1038/ni.1985 pubmed: 21245903
Franchi L, Muñoz-Planillo R, Núñez G (2012) Sensing and reacting to microbes via the inflammasomes. Nat Immunol 13:325–332. https://doi.org/10.1038/ni.2231
doi: 10.1038/ni.2231 pubmed: 22430785 pmcid: 3449002
Latz E, Xiao TS, Stutz A (2013) Activation and regulation of the inflammasomes. Nat Rev Immunol 13:397–411. https://doi.org/10.1038/nri3452
doi: 10.1038/nri3452 pubmed: 23702978
Stutz A, Kolbe C-C, Stahl R et al (2017) NLRP3 inflammasome assembly is regulated by phosphorylation of the pyrin domain. J Exp Med 214:1725–1736. https://doi.org/10.1084/jem.20160933
doi: 10.1084/jem.20160933 pubmed: 28465465 pmcid: 5460996
Marleaux M, Anand K, Latz E, Geyer M (2020) Crystal structure of the human NLRP9 pyrin domain suggests a distinct mode of inflammasome assembly. FEBS Lett 594:2383–2395. https://doi.org/10.1002/1873-3468.13865
doi: 10.1002/1873-3468.13865 pubmed: 32542665
Fernandes-Alnemri T, Wu J, Yu J-W et al (2007) The pyroptosome: a supramolecular assembly of ASC dimers mediating inflammatory cell death via caspase-1 activation. Cell Death Differ 14:1590–1604. https://doi.org/10.1038/sj.cdd.4402194
doi: 10.1038/sj.cdd.4402194 pubmed: 17599095
Hochheiser IV, Behrmann H, Hagelueken G et al (2022) Directionality of PYD filament growth determined by the transition of NLRP3 nucleation seeds to ASC elongation. Sci Adv 8:eabn7583. https://doi.org/10.1126/sciadv.abn7583
Tropea JE, Cherry S, Waugh DS (2009) Expression and purification of soluble His6-tagged TEV protease. In: Doyle SA (ed) High throughput protein expression and purification: methods and protocols. Humana Press, Totowa, pp 297–307
doi: 10.1007/978-1-59745-196-3_19
Venegas C, Kumar S, Franklin BS et al (2017) Microglia-derived ASC specks cross-seed amyloid-β in Alzheimer’s disease. Nature 552:355–361. https://doi.org/10.1038/nature25158
doi: 10.1038/nature25158 pubmed: 29293211

Auteurs

Inga V Hochheiser (IV)

Institute of Structural Biology, University Hospital Bonn, University of Bonn, Bonn, Germany.

Matthias Geyer (M)

Institute of Structural Biology, University Hospital Bonn, University of Bonn, Bonn, Germany. matthias.geyer@uni-bonn.de.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Caspases › Caspases initiatrices › Caspase-1 › An Assay for the Seeding of Homotypic Pyrin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › An Assay for the Seeding of Homotypic Pyrin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Protéines NLR › Protéine-3 de la famille des NLR contenant un domaine pyrine › An Assay for the Seeding of Homotypic Pyrin...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › An Assay for the Seeding of Homotypic Pyrin...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Motifs et domaines d'intéraction protéique › Superfamille des domaines de mort › Domaine pyrine › An Assay for the Seeding of Homotypic Pyrin...