The meiotic topoisomerase VI B subunit (MTOPVIB) is essential for meiotic DNA double-strand break formation in barley (Hordeum vulgare L.).
Barley
MTOPVIB
Meiosis
Meiotic DSB
Meiotic recombination
Meiotic spindle
Journal
Plant reproduction
ISSN: 2194-7961
Titre abrégé: Plant Reprod
Pays: Germany
ID NLM: 101602701
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
14
02
2022
accepted:
31
05
2022
pubmed:
30
6
2022
medline:
3
3
2023
entrez:
29
6
2022
Statut:
ppublish
Résumé
In barley (Hordeum vulgare), MTOPVIB is critical for meiotic DSB and accompanied SC and CO formation while dispensable for meiotic bipolar spindle formation. Homologous recombination during meiosis assures genetic variation in offspring. Programmed meiotic DNA double-strand breaks (DSBs) are repaired as crossover (CO) or non-crossover (NCO) during meiotic recombination. The meiotic topoisomerase VI (TopoVI) B subunit (MTOPVIB) plays an essential role in meiotic DSB formation critical for CO-recombination. More recently MTOPVIB has been also shown to play a role in meiotic bipolar spindle formation in rice and maize. Here, we describe a meiotic DSB-defective mutant in barley (Hordeum vulgare L.). CRISPR-associated 9 (Cas9) endonuclease-generated mtopVIB plants show complete sterility due to the absence of meiotic DSB, synaptonemal complex (SC), and CO formation leading to the occurrence of univalents and their unbalanced segregation into aneuploid gametes. In HvmtopVIB plants, we also frequently found the bi-orientation of sister kinetochores in univalents during metaphase I and the precocious separation of sister chromatids during anaphase I. Moreover, the near absence of polyads after meiosis II, suggests that despite being critical for meiotic DSB formation in barley, MTOPVIB seems not to be strictly required for meiotic bipolar spindle formation.
Identifiants
pubmed: 35767067
doi: 10.1007/s00497-022-00444-5
pii: 10.1007/s00497-022-00444-5
pmc: PMC9957907
doi:
Substances chimiques
DNA topoisomerase VI
EC 5.99.1.-
DNA Topoisomerases, Type II
EC 5.99.1.3
Archaeal Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-15Informations de copyright
© 2022. The Author(s).
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