PbrCalS5, a callose synthase protein, is involved in pollen tube growth in Pyrus bretschneideri.
CalS
Callose
Evolution
Pear
Pollen tube
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
29 Jun 2022
29 Jun 2022
Historique:
received:
19
03
2022
accepted:
27
05
2022
entrez:
29
6
2022
pubmed:
30
6
2022
medline:
2
7
2022
Statut:
epublish
Résumé
Identification of CalS genes in seven Rosaceae species and functional characterization of PbrCalS5 in pear pollen tube growth by regulating callose deposition. Callose exists widely in angiosperms and has significant functions in a range of developmental processes. Callose is synthesized by callose synthase (CalS). However, the members of the callose synthase gene family and their evolutionary profiles, along with their biological functions, in species of the Rosaceae remain unknown. In this study, a total of 69 members of the CalS gene family in seven Rosaceae species (Fragaria vesca, Malus × domestica, Prunus avium, Pyrus bretschneideri, Prunus mume, Prunus persica and Rubus occidentalis) were identified and divided into six clades. Different types of gene duplication events contributed to the expansions of the CalS gene family in the seven species, with purifying selection playing a key role in the evolution of the CalS genes. Tissue-specific expression patterns analysis revealed that PbrCalS5 was highly expressed in the pear pollen tube and was selected for further functional analysis. Subcellular localization indicated that PbrCalS5 was localized in the plasma membrane and cell wall. Antisense oligodeoxynucleotide (AS-ODN) assays resulted in the inhibition of PbrCalS5 expression, leading to the decreased callose deposition in the pollen tube wall and subsequent inhibition of pear pollen tube growth. These results provide the theoretical basis for exploring the functional roles of CalS genes in pear pollen tube growth.
Identifiants
pubmed: 35767158
doi: 10.1007/s00425-022-03931-1
pii: 10.1007/s00425-022-03931-1
doi:
Substances chimiques
Plant Proteins
0
Glucosyltransferases
EC 2.4.1.-
1,3-beta-glucan synthase
EC 2.4.1.34
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
22Subventions
Organisme : National Natural Science Foundation of China
ID : 32172543
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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