Mesorhizobium ventifaucium sp. nov. and Mesorhizobium escarrei sp. nov., two novel root-nodulating species isolated from Anthyllis vulneraria.
Mesorhizobium sp. nov.
Phylogeny
Rhizobia
Symbiosis
Symbiovar
Taxonomy
Journal
Systematic and applied microbiology
ISSN: 1618-0984
Titre abrégé: Syst Appl Microbiol
Pays: Germany
ID NLM: 8306133
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
24
03
2022
revised:
23
05
2022
accepted:
07
06
2022
pubmed:
15
7
2022
medline:
24
9
2022
entrez:
14
7
2022
Statut:
ppublish
Résumé
Ten mesorhizobial strains isolated from root-nodules of Anthyllis vulneraria by trapping using soils from southern France were studied to resolve their taxonomy. Their 16S rDNA sequences were identical and indicated that they are affiliated to the genus Mesorhizobium within the group M. prunaredense/M. delmotii/M. temperatum/M. mediterraneum/M. wenxiniae and M. robiniae as the closest defined species. Their evolutionary relationships with validated species were further characterized by multilocus sequence analysis (MLSA) using 4 protein-coding housekeeping genes (recA, atpD, glnII and dnaK), that divides the strains in two groups, and suggest that they belong to two distinct species. These results were well-supported by MALDI-TOF mass spectrometry analyses, wet-lab DNA-DNA hybridization (≤58%), and genome-based species delineation methods (ANI < 96%, in silico DDH < 70%), confirming their affiliation to two novel species. Based on these differences, Mesorhizobium ventifaucium (STM4922
Identifiants
pubmed: 35834932
pii: S0723-2020(22)00048-0
doi: 10.1016/j.syapm.2022.126341
pii:
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Ribosomal
0
RNA, Ribosomal, 16S
0
Soil
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126341Informations de copyright
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