Identification and characterization of a new geminivirus from soybean plants and determination of V2 as a pathogenicity factor and silencing suppressor.
Genetic variability
Pathogenicity
RNA silencing suppressor
Soybean geminivirus A (SGVA)
V2 protein
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
22 Jul 2022
22 Jul 2022
Historique:
received:
22
03
2022
accepted:
04
07
2022
entrez:
22
7
2022
pubmed:
23
7
2022
medline:
27
7
2022
Statut:
epublish
Résumé
Soybean is one of the four major crops in China. The occurrence of viruses in soybean causes significant economic losses. In this study, the soybean leaves from stay-green plants showing crinkle were collected for metatranscriptomic sequencing. A novel geminivirus, tentatively named soybean geminivirus A (SGVA), was identified in soybean stay-green plants. Sequence analysis of the full-length SGVA genome revealed a genome of 2762 nucleotides that contain six open reading frames. Phylogenetic analyses revealed that SGVA was located adjacent to the clade of begomoviruses in both the full genome-based and C1-based phylogenetic tree, while in the CP-based phylogenetic tree, SGVA was located adjacent to the clade of becurtoviruses. SGVA was proposed as a new recombinant geminivirus. Agroinfectious clone of SGVA was constructed. Typical systemic symptoms of curly leaves were observed at 11 dpi in Nicotiana benthamiana plants and severe dwarfism was observed after 3 weeks post inoculation. Expression of the SGVA encoded V2 and C1 proteins through a potato virus X (PVX) vector caused severe symptoms in N. benthamiana. The V2 protein inhibited local RNA silencing in co-infiltration assays in GFP transgenic 16C N. benthamiana plants. Further study revealed mild symptoms in N. benthamiana plants inoculated with SGVA-ZZ This work identified a new geminivirus in soybean stay-green plants and determined V2 as a pathogenicity factor and silencing suppressor.
Sections du résumé
BACKGROUND
BACKGROUND
Soybean is one of the four major crops in China. The occurrence of viruses in soybean causes significant economic losses.
RESULTS
RESULTS
In this study, the soybean leaves from stay-green plants showing crinkle were collected for metatranscriptomic sequencing. A novel geminivirus, tentatively named soybean geminivirus A (SGVA), was identified in soybean stay-green plants. Sequence analysis of the full-length SGVA genome revealed a genome of 2762 nucleotides that contain six open reading frames. Phylogenetic analyses revealed that SGVA was located adjacent to the clade of begomoviruses in both the full genome-based and C1-based phylogenetic tree, while in the CP-based phylogenetic tree, SGVA was located adjacent to the clade of becurtoviruses. SGVA was proposed as a new recombinant geminivirus. Agroinfectious clone of SGVA was constructed. Typical systemic symptoms of curly leaves were observed at 11 dpi in Nicotiana benthamiana plants and severe dwarfism was observed after 3 weeks post inoculation. Expression of the SGVA encoded V2 and C1 proteins through a potato virus X (PVX) vector caused severe symptoms in N. benthamiana. The V2 protein inhibited local RNA silencing in co-infiltration assays in GFP transgenic 16C N. benthamiana plants. Further study revealed mild symptoms in N. benthamiana plants inoculated with SGVA-ZZ
CONCLUSIONS
CONCLUSIONS
This work identified a new geminivirus in soybean stay-green plants and determined V2 as a pathogenicity factor and silencing suppressor.
Identifiants
pubmed: 35869422
doi: 10.1186/s12870-022-03745-z
pii: 10.1186/s12870-022-03745-z
pmc: PMC9308217
doi:
Substances chimiques
Virulence Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
362Informations de copyright
© 2022. The Author(s).
Références
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Viruses. 2018 Sep 11;10(9):
pubmed: 30208627
Front Plant Sci. 2020 Jun 19;11:835
pubmed: 32636860
Arch Virol. 2021 Sep;166(9):2633-2648
pubmed: 34231026
J Exp Bot. 2011 May;62(9):3093-102
pubmed: 21398430
Arch Virol. 2017 Jun;162(6):1799-1803
pubmed: 28243802
DNA Res. 2011 Feb;18(1):53-63
pubmed: 21217129
Virus Evol. 2017 Mar 02;3(1):vex005
pubmed: 28458915
J Virol Methods. 2019 Jun;268:24-31
pubmed: 30890330
Genome Res. 1999 Sep;9(9):868-77
pubmed: 10508846
Mol Plant Microbe Interact. 2020 Mar;33(3):412-422
pubmed: 31841359
Plant Dis. 2008 Dec;92(12):1709
pubmed: 30764308
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Plant Cell Physiol. 2011 Sep;52(9):1501-14
pubmed: 21771864
Plant J. 2002 Aug;31(3):375-83
pubmed: 12164816
Mol Plant Microbe Interact. 2020 Apr;33(4):637-652
pubmed: 31935338
Adv Virus Res. 2014;90:355-90
pubmed: 25410106
Front Plant Sci. 2020 Sep 17;11:527787
pubmed: 33042171
Arch Virol. 2020 Jan;165(1):257-260
pubmed: 31773325
Front Plant Sci. 2020 May 19;11:591
pubmed: 32508858
J Gen Virol. 2017 Feb;98(2):131-133
pubmed: 28284245
Front Microbiol. 2021 Sep 13;12:680658
pubmed: 34589062
Trends Plant Sci. 2006 May;11(5):209-12
pubmed: 16616578
J Virol. 2008 Jan;82(2):957-65
pubmed: 17977971
Virology. 2010 Jan 5;396(1):85-93
pubmed: 19896687
J Gen Virol. 2009 Jun;90(Pt 6):1539-1547
pubmed: 19264617
Arch Virol. 2013 Feb;158(2):457-62
pubmed: 23053525
Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8029-33
pubmed: 1896448
J Virol Methods. 2017 Nov;249:126-129
pubmed: 28887190