Speed variations of bacterial replisomes.
DNA replication
E. coli
physics of living systems
replication completion
replication forks
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
25 07 2022
25 07 2022
Historique:
received:
26
11
2021
accepted:
13
07
2022
pubmed:
26
7
2022
medline:
20
8
2022
entrez:
25
7
2022
Statut:
epublish
Résumé
Replisomes are multi-protein complexes that replicate genomes with remarkable speed and accuracy. Despite their importance, their dynamics is poorly characterized, especially in vivo. In this paper, we present an approach to infer the replisome dynamics from the DNA abundance distribution measured in a growing bacterial population. Our method is sensitive enough to detect subtle variations of the replisome speed along the genome. As an application, we experimentally measured the DNA abundance distribution in
Identifiants
pubmed: 35877175
doi: 10.7554/eLife.75884
pii: 75884
pmc: PMC9385209
doi:
pii:
Substances chimiques
DNA, Bacterial
0
Escherichia coli Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022, Bhat et al.
Déclaration de conflit d'intérêts
DB, SH, CP, YY, SP No competing interests declared
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