Global chromatin mobility induced by a DSB is dictated by chromosomal conformation and defines the HR outcome.
S. cerevisiae
chromatin dynamics
chromosome organization
chromosomes
double-strand break
gene expression
genetics
genomics
homologous recombination
yeast
γ-H2A(X)
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
20 09 2022
20 09 2022
Historique:
received:
19
02
2022
accepted:
08
09
2022
entrez:
20
9
2022
pubmed:
21
9
2022
medline:
24
9
2022
Statut:
epublish
Résumé
Repair of DNA double-strand breaks (DSBs) is crucial for genome integrity. A conserved response to DSBs is an increase in chromatin mobility that can be local, at the site of the DSB, or global, at undamaged regions of the genome. Here, we address the function of global chromatin mobility during homologous recombination (HR) of a single, targeted, controlled DSB. We set up a system that tracks HR in vivo over time and show that two types of DSB-induced global chromatin mobility are involved in HR, depending on the position of the DSB. Close to the centromere, a DSB induces global mobility that depends solely on H2A(X) phosphorylation and accelerates repair kinetics, but is not essential. In contrast, the global mobility induced by a DSB away from the centromere becomes essential for HR repair and is triggered by homology search through a mechanism that depends on H2A(X) phosphorylation, checkpoint progression, and Rad51. Our data demonstrate that global mobility is governed by chromosomal conformation and differentially coordinates repair by HR.
Identifiants
pubmed: 36125964
doi: 10.7554/eLife.78015
pii: 78015
pmc: PMC9489209
doi:
pii:
Substances chimiques
Chromatin
0
DNA
9007-49-2
Banques de données
Dryad
['10.5061/dryad.931zcrjn1']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022, García Fernández, Almayrac et al.
Déclaration de conflit d'intérêts
FG, EA, ÀC, RB, YK, MB, EF No competing interests declared
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