An in vitro fermentation model to study the impact of bacteriophages targeting Shiga toxin-encoding Escherichia coli on the colonic microbiota.
Journal
NPJ biofilms and microbiomes
ISSN: 2055-5008
Titre abrégé: NPJ Biofilms Microbiomes
Pays: United States
ID NLM: 101666944
Informations de publication
Date de publication:
26 09 2022
26 09 2022
Historique:
received:
07
01
2022
accepted:
02
09
2022
entrez:
27
9
2022
pubmed:
28
9
2022
medline:
1
10
2022
Statut:
epublish
Résumé
Lytic bacteriophages are considered safe for human consumption as biocontrol agents against foodborne pathogens, in particular in ready-to-eat foodstuffs. Phages could, however, evolve to infect different hosts when passing through the gastrointestinal tract (GIT). This underlines the importance of understanding the impact of phages towards colonic microbiota, particularly towards bacterial families usually found in the colon such as the Enterobacteriaceae. Here we propose in vitro batch fermentation as model for initial safety screening of lytic phages targeting Shiga toxin-producing Escherichia coli (STEC). As inoculum we used faecal material of three healthy donors. To assess phage safety, we monitored fermentation parameters, including short chain fatty acid production and gas production/intake by colonic microbiota. We performed shotgun metagenomic analysis to evaluate the outcome of phage interference with colonic microbiota composition and functional potential. During the 24 h incubation, concentrations of phage and its host were also evaluated. We found the phage used in this study, named E. coli phage vB_EcoS_Ace (Ace), to be safe towards human colonic microbiota, independently of the donors' faecal content used. This suggests that individuality of donor faecal microbiota did not interfere with phage effect on the fermentations. However, the model revealed that the attenuated STEC strain used as phage host perturbed the faecal microbiota as based on metagenomic analysis, with potential differences in metabolic output. We conclude that the in vitro batch fermentation model used in this study is a reliable safety screening for lytic phages intended to be used as biocontrol agents.
Identifiants
pubmed: 36163472
doi: 10.1038/s41522-022-00334-8
pii: 10.1038/s41522-022-00334-8
pmc: PMC9512901
doi:
Substances chimiques
Shiga Toxin
75757-64-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
74Informations de copyright
© 2022. The Author(s).
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Front Cell Infect Microbiol. 2013 Jun 06;3:20
pubmed: 23761050
Cell Host Microbe. 2019 Jun 12;25(6):803-814.e5
pubmed: 31175044
Antibiotics (Basel). 2018 Nov 16;7(4):
pubmed: 30453470
Appl Microbiol Biotechnol. 1999 Dec;53(1):108-14
pubmed: 10645630
FEMS Microbiol Ecol. 2021 Aug 3;97(8):
pubmed: 34329454
Nat Methods. 2018 Nov;15(11):962-968
pubmed: 30377376
J Microbiol Methods. 2013 Nov;95(2):167-74
pubmed: 23994646
Food Funct. 2014 Jun;5(6):1113-24
pubmed: 24803111
Epidemiol Infect. 2019 Jan;147:e236
pubmed: 31364563
Int J Food Sci Nutr. 2014 Feb;65(1):79-88
pubmed: 23941288
Cell Host Microbe. 2017 Dec 13;22(6):801-808.e3
pubmed: 29174401
Virol J. 2020 Jan 7;17(1):3
pubmed: 31910855
Environ Microbiol. 2020 Nov;22(11):4863-4875
pubmed: 33001550
Food Res Int. 2020 Feb;128:108791
pubmed: 31955758
Gut Microbes. 2018;9(5):391-399
pubmed: 29517960
J Microbiol Methods. 2016 Oct;129:109-116
pubmed: 27498348
Curr Opin Biotechnol. 2021 Apr;68:96-103
pubmed: 33186799
Benef Microbes. 2016 Feb;7(1):119-133
pubmed: 26615853
Int J Mol Sci. 2019 Apr 18;20(8):
pubmed: 31003566
Antibiotics (Basel). 2019 Jun 05;8(2):
pubmed: 31195679
Front Microbiol. 2016 Jun 08;7:825
pubmed: 27375566
Sci Rep. 2019 Apr 30;9(1):6643
pubmed: 31040333
Sci Rep. 2016 Dec 15;6:39235
pubmed: 27976713
J Microbiol Methods. 2014 Dec;107:1-7
pubmed: 25194233
PLoS One. 2016 Aug 02;11(8):e0160533
pubmed: 27483470
Mol Nutr Food Res. 2018 Jun;62(12):e1800232
pubmed: 29710405
Front Microbiol. 2017 Mar 23;8:467
pubmed: 28386250
Appl Environ Microbiol. 1988 Nov;54(11):2750-5
pubmed: 3214155
Appl Environ Microbiol. 2012 Jan;78(1):58-69
pubmed: 22020516
Z Gastroenterol. 2001 Nov;39(11):911-8
pubmed: 11778150
Res Microbiol. 2011 Oct;162(8):798-806
pubmed: 21782936
J Am Coll Nutr. 2009 Dec;28(6):657-66
pubmed: 20516265
Front Microbiol. 2018 Aug 14;9:1835
pubmed: 30154767
BMC Microbiol. 2012 Aug 01;12:160
pubmed: 22853739
J Biosci. 2019 Oct;44(5):
pubmed: 31719224
FEMS Microbiol Rev. 2020 Nov 24;44(6):684-700
pubmed: 32472938
J Control Release. 2020 Oct 10;326:106-119
pubmed: 32569705
Front Microbiol. 2020 Jan 31;11:58
pubmed: 32082285
ISME J. 2019 Sep;13(9):2306-2318
pubmed: 31089259
Front Microbiol. 2019 Jan 23;9:3328
pubmed: 30761120
PLoS One. 2014 Nov 26;9(11):e113864
pubmed: 25426858
J Microbiol Methods. 2015 Oct;117:78-84
pubmed: 26222994
Crit Rev Microbiol. 2020 Feb;46(1):78-99
pubmed: 32091280
PeerJ. 2016 Sep 13;4:e2423
pubmed: 27672499
PLoS Med. 2015 Dec 03;12(12):e1001923
pubmed: 26633896